The catalytic subunit of the H+/K(+)-transporting ATPase (EC 3.6.1.3) has 62% identity to the alpha, or catalytic subunit, of the Na+/K(+)-transporting ATPase (EC 3.6.1.37); however, a homologous beta subunit was unknown until recently. Removal of the carbohydrate from purified hog H+/K(+)ATPase vesicles reveals a 35-kDa peptide that, when fragmented with protease V8, gives sequences homologous...

H+-translocating, Mg2+-ATPase was solubilized from vacuolar membranes of Saccharomyces cerevisiae with the zwitterionic detergent N-tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate and purified by glycerol density gradient centrifugation. Partially purified vacuolar membrane H+-ATPase, which had a specific activity of 18 units/mg of protein, was separated almost completely from acid phospha...

The plasma membrane H+-ATPase plays an important role in the plant response to nutrient and environmental stresses. However, the involvement of plant root plasma membrane H+-ATPase in adaptation to phosphate (P) starvation is not yet fully elucidated. In this study, experiments were performed with soybean roots in low-P nutrient solution (10 microM). Treatment with fusicoccin, an activator of t...

OBJECTIVE To explore the functional role of vacuolar H(+)-ATPase in the pH regulation of epididymal fluid and its effect on sperm motility. DESIGN Experimental study. SETTING Physiology laboratory in a university. ANIMAL(S) Immature male Sprague-Dawley rats. INTERVENTION(S) The H(+)-ATPase inhibitor was applied to the primary culture of epididymal cells. MAIN OUTCOME MEASURE(S) The in...

The structure, ultrastructure and osmoregulatory function of the branchial chamber were investigated in Palaemon elegans (Rathke, 1837) by light microscope, electron microscope and immunohistochemistry methods, respectively. Each chamber possesses a branchiostegite, 3 epipodites and 8 phyllobranchiate gills. The lamellae are attached along the two outer surfaces of the triangular gill axis. Ult...

Abstract Background and Aims The ATP6V0A4 gene is localized to chromosome 7q33∼34 encodes for vacuolar H+-ATPase (V-ATPase) α4 subunit. Decreased V-ATPase function due mutations in the genes could cause H+ excretion defect of α-intercalated cells renal collecting duct, resulting hereditary distal tubular acidosis (dRTA). However, crucially, no gain-of-function have been reported previously. Thi...

Rhythmic daily changes in the Na,K-ATPase activity have been previously described for rat kidney cortex, showing two peaks: at 0900 h and 2100 h, and two valleys: at 1500 h and 0100 h -0300 h. The oscillations in Na,K-ATPase activity are produced by an inhibitor, which binds the enzyme and is present in the rat blood plasma at valley times and absent or at very low concentrations at peak times....

The plasma membrane proton pump (H+-ATPase) energizes solute uptake by secondary transporters. Wild-type Arabidopsis plasma membrane H+-ATPase (AHA2) and truncated H+-ATPases lacking 38, 51, 61, 66, 77, 92, 96, and 104 C-terminal amino acids were produced in yeast. All AHAS species were correctly targeted to the yeast plasma membrane and, in addition, accumulated in interna1 membranes. Removal ...

The chromaffin granule ATPase mediates an inwardly directed transport of H+ against concentration gradients, thereby forming and maintaining an electrochemical transmembrane H+ gradient. The kinetics of this ATPase, its activity modulation by changes in electrochemical H+ gradients, and the stoichiometry between H+ transport and ATP hydrolysis were studied in intact bovine chromaffin granules, ...