Here, we demonstrate that the metabolism of glyceryl trinitrate (GTN) to nitric oxide (NO) occurs not only in bovine aortic smooth muscle cells (SMCs) but also in endothelial cells (ECs) and that this biotransformation is enhanced by pretreatment with Escherichia coli lipopolysaccharide (LPS). Two bioassay systems were used: inhibition of platelet aggregation and measurement of cGMP after stimu...

Previous studies have shown that nitroglycerin (GTN) tolerance is closely related to an oxidative stress-induced decrease in activity of mitochondrial isoforms of aldehyde dehydrogenase (ALDH-2), and prolonged GTN treatment causes endothelial dysfunction. Asymmetric dimethylarginine (ADMA), a major endogenous NO synthase (NOS) inhibitor, could inhibit NO production and induce oxidative stress i...

OBJECTIVE This study was conducted to compare the tocolytic efficacy of glyceryltrinitrate (GTN) with that of magnesium sulfate (MgSO4) and to investigate serum nitric oxide metabolites before and after tocolysis. MATERIAL AND METHODS In total, 48 women between 27 and 34 weeks' gestation with threatened preterm labor and intact membranes were randomly allocated to receive either GTN or MgSO4 ...

  Abstract: Gestational Trophoblastic Neoplasm (GTN) is among rare human tumors which can be observed with widespread metastasis. Two young patients with emergent neurologic symptoms with no gynecological problems are presented in this report. GTN was later diagnosed in both cases with brain metastasis. The first case: A 22 years old patient, admitted to the infectious disease ward, with probab...

Pharmacokinetic analysis of nitroglycerin (GTN) has been hampered by the lack of a sensitive and specific method for measuring GTN in blood. Therefore, we examined the appearance of GTN in blood after administering 0.6 mg sublingually in 10 studies of normal volunteers. We used a gas-liquid chromatographic method with electron-capture detection and isosorbide dinitrate as the internal standard....

Nitroglycerin (glyceryl trinitrate, GTN), originally manufactured by Alfred Nobel, has been used to treat angina and heart failure for over 130 years. However, the molecular mechanism of GTN biotransformation has remained a mystery and it is not well understood why "tolerance" (i.e., loss of clinical efficacy) manifests over time. Here we purify a nitrate reductase that specifically catalyzes t...

[reaction--see text] Nitric oxide (NO) is an important biological messenger molecule. Nitrates, including nitroglycerin (GTN), are clinically important vasodilators believed to be biotransformed in vivo to NO, a 3e(-) reduction. Molybdenum hydrotris-(3,5-dimethyl-1-pyrazolyl) borate complex (MoTPB) was shown to be an efficient catalyst of GTN degradation, with triphenylphosphine (Ph(3)P) acting...

High blood pressure (BP) is common in acute stroke and is independently associated with a poor outcome. Lowering BP might improve outcome if it did not adversely affect cerebral blood flow (CBF) or cerebral perfusion pressure. We investigated the effect of glyceryl trinitrate ([GTN] an NO donor) on quantitative CBF, BP, and cerebral perfusion pressure in patients with recent stroke. Eighteen pa...

early recognition of gestational trophoblastic neoplasm (gtn) will maximize the chances of cure with chemotherapy but some patients present with many different symptoms months or even years after the causative pregnancy making diagnosis difficult. clinicians should be aware of the possibility of gtn in any reproductive age woman with bizarre central nervous system, gastrointestinal, pulmonary s...

Intra-observer and inter-study reproducibility of brachial artery flow-mediated dilatation (FMD) and glyceryl trintrate (GTN)-mediated dilatation by CMR at 3Tesla were assessed. Coefficients of variation (CoV) were calculated using the root mean square method. The intra-observer CoV, assessed in 15 subjects, was 15% for both FMD and GTN response. The inter-study CoV, assessed in 17 subjects, wa...