I The phyto-estrogens formononet in (7-hydroxy-4 methoxylsoflavone) and biochanin A (5-7-dlhydroxy-4-methoxylsof lavone) were independently incubated in vitro at 39~ in bovine rumen fluid from a fistulated steer receiving an alfalfa hay diet. Formononet in was incubated in studies 1 and 2, whereas biochanin A was incubated in s tudy 3. The isoflavones were separated and quantified by high perfo...

CcaR is a positive-acting transcriptional regulator involved in cephamycin C and clavulanic acid biosynthesis in Streptomyces clavuligerus. Previous sequence analyses of the ccaR gene revealed two possible start codons, an ATG, and a GTG located in-frame 18 bp downstream of the ATG. To determine the true start codon, ccaR was expressed, either from the GTG or ATG codon, in Escherichia coli. A p...

AIM To evaluate the role of whole genome comparative genomic hybridisation microarray (array-CGH) in detecting genomic imbalances as compared to conventional karyotype (GTG-analysis) or myeloma specific fluorescence in situ hybridisation (FISH) panel in a diagnostic setting for plasma cell dyscrasia (PCD). METHODS A myeloma-specific interphase FISH (i-FISH) panel was carried out on CD138 PC-e...

Five repetitive-element PCR (rep-PCR) techniques [primer sets ERIC1R-ERIC2 and REP1R-REP2I and primers ERIC2, BOXA1R, and (GTG)5] were evaluated for the discrimination of Salmonella enterica isolates at the serotype level. On the basis of number, even distribution over the whole fingerprint, and clarity of bands in the fingerprints, the enterobacterial repetitive intergenic consensus (ERIC) pri...

abstract  background: mosaic form of turner syndrome that represented by two or more  cell lines in an affected individual, often has limitation for detection with classical  cytogenetic methods. the present study was carried out to compare the efficiency of  interphase fluorescence in situ hybridisation (fish) and cytogenetic techniques in  detection of mosaic form of turner syndrome.  methods...

Introduction Reproductive failure (RF) includes a variety of problems such as infertility, recurrent miscarriages, stillbirths, the birth children with multiple defects, and/or mental retardation. Chromosomal aberrations in one partners (in 2-8%) are considered to be causes reduced fertility couples reproductive age. Aim The aim this study was evaluate effect chromosomal abnormalities families ...

OBJECTIVE To evaluate the effect of the consumption of green tea on components of MS in the elderly. DESIGN Intervention study. SETTING The sample was selected from the Geriatric Service of Hospital São Lucas of Pontifical Catholic University of Rio Grande do Sul. PARTICIPANTS 45 elderly with MS were enrolled and allocated into two groups: green tea group (GTG, n= 24), who drank green tea...

In this paper we propose new three-input XOR and three-input XNOR gate based on generalized threshold gate (GTG) topology. The GTG topology is main part is monostable-bistable logic element (MOBILE). The proposed gates use fewer elements count in comparison with other implementations which utilize MOBILE as a main structure. By exploiting the new three-input XOR gate and a carry generator, we p...