Subgingival plaque samples obtained from human subjects with periodontitis, shown to include previously uncultivable members of the phylum Synergistetes, were used to inoculate Cooked Meat Medium (CMM). The presence of Cluster A (uncultivable) Synergistetes was monitored by fluorescent in situ hybridization (FISH) and quantitative PCR (Q-PCR). Cluster A Synergistetes were found to grow in CMM i...

The present paper aims to study the crack propagating behavior of a stiffened plate under tensile and bending displacement load loads. extended finite element method (XFEM) is used analyze residual ultimate strength plates with central crack. quasi-static growth process simulated by software ABAQUS. validity grid validated numerical comparing fatigue rate round compact tension specimen (RCT) re...

A technique for culturing two species of trypanosomatid flagellates on blood-agar plates is described. Herpetomonas megaseliae and Crithidia harmosa were grown as discrete clone colonies on plates prepared in sterile petri dishes with a medium derived from that of Tobie et al. (1950, J Parasitol 36: 48-54). Plates were inoculated with 0.01 ml flagellate suspension and the inoculum was spread ev...

Hepatocytes were grown in chemically defined hepatocyte growth medium (HGM) containing hepatocyte growth factor (HGF) and epidermal growth factor (EGF) on collagen-coated polystyrene beads in roller bottle cultures, forming clusters of beads, and proliferating hepatocytes and nonparenchymal cells, including fenestrated endothelium-forming vascular structures. Desmin-positive cells surrounded he...

—The evolution of scutes in thyreophoran dinosaurs, based on Scutellosaurus, Scelidosaurus, Stegosaurus, and several ankylosaurs, began with small rounded or ovoid structures that typically had slight, anteroposteriorly oriented keels. These scutes were elaborated in two general and overlapping ways: they could flare laterally and asymmetrically beneath the keels that mark the anteroposterior a...

Achieving high fidelity chemical synthesis on glass plates has become increasingly important, since glass plates are substrates widely used for miniaturized chemical and biochemical reactions and analyses. DNA chips can be directly prepared by synthesizing oligonucleotides on glass plates, but the characterization of these micro-syntheses has been limited by the sub-picomolar amount of material...

Background: Although BCG is the most widely administered vaccine in the world, there have never been as many cases of TB as there are now. Globally, more than 8.8 million people developed active TB and 1.4 million—many of them—died in 2010. It is estimated that half of pulmonary TB cases arise from latent Mtb infection, making the study of latency and reactivation of utmost importance. Methods:...

background solid organ transplantation patients are at high risk for opportunistic air-borne fungal infections due to using the potent immunosuppressive agents. objectives the current study aimed to qualitatively and quantitatively evaluate the fungal flora present in the air of kidney transplant unit of baqiyatallah hospital. materials and methods in this prospective study, air samples from pa...

Results Consequently, the imposed convex-wise, asymmetrically concentrated cyclical loads on the adjacent immature vertebral end plates and posterior elements of the spine lead to asymmetrical vertebral growth. More specifically the loading on the two facet-joins asymmetrically increases during the day, as the wedged disc space narrows due to expelled water and it asymmetrically decreases durin...