To study the effects of acute ammonia stress on poisoning reaction hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatu ), 300 healthy juveniles with an initial body weight 51.4 ± 2.57 g were selected for experiment using a half-lethal concentration 24, 48, 72, and 96 h triplicate. The results show that (1) concentrations 39.5 mg/L 24 h, 27.3 48 26.5 72 25.0 safe was 2.50 mg/L. (2) bio...

Viral infection is a great challenge in healthcare and agriculture. The Singapore grouper iridovirus (SGIV) is highly infectious to numerous marine fishes and increasingly threatens mariculture and wildlife conservation. SGIV intervention is not available because little is known about key players and their precise roles in SGVI infection. Here we report the precise role of VP088 as a key player...

Thirunavukkarasu Periyasamy 1, Ming-Wei Lu 1, 2, * 5 1 Laboratory of Molecular Virology and Immunology, Department of Aquaculture, The College of 6 Life Science, National Taiwan Ocean University, No. 2, Beining Road, Keelung 202, Taiwan; 7 [email protected] 8 2 Center of Excellence for the Oceans, National Taiwan Ocean University, No. 2, Beining Road, 9 Keelung 202, Taiwan; [email protected]...

This study was conducted to investigate the effects of Curcuma zedoaria and Zingiber zerumbet on non-specific immune responses of grouper (Epinephelus coioides). Fish were fed an experimental diet containing 0, 0.5, 1.0, 2.5 g/kg of C. zedoaria and Z. zerumbet mixed diets twice daily for two weeks. Non-specific immune parameters such as respiratory burst activity, reactive oxygen species, phago...

Sparus aurata larvae infected by viral nervous necrosis were collected from an aquaculture fish farm. The isolated viral genome, composed of two segments (RNA1 and RNA2), was sequenced and analyzed comparatively. Phylogenetic tree analyses revealed that the isolated strain is a reassortant, exhibiting a red-spotted grouper nervous necrosis virus (RGNNV)-type RNA1 and a striped jack nervous necr...

To address patterns of genetic connectivity in a mass-aggregating marine fish, we analyzed genetic variation in mitochondrial DNA (mtDNA), microsatellites, and single nucleotide polymorphisms (SNPs) for Nassau grouper (Epinephelus striatus). We expected Nassau grouper to exhibit genetic differentiation among its subpopulations due to its reproductive behavior and retentive oceanographic conditi...

Diplectanid monogeneans are gill parasites that can infect fish in huge numbers and thus become harmful, especially in maricultured fish. It is therefore useful to have taxonomic tools, such as keys, to identify species. The following diplectanid species from groupers of the Mediterranean Sea were studied: five species of Pseudorhabdosynochus Yamaguti, 1958, including P. riouxi (Oliver, 1986) K...

Understanding of species interactions within mesophotic coral ecosystems (MCEs; ~ 30-150 m) lags well behind that for shallow coral reefs. MCEs are often sites of fish spawning aggregations (FSAs) for a variety of species, including many groupers. Such reproductive fish aggregations represent temporal concentrations of potential prey that may be drivers of habitat use by predatory species, incl...

BACKGROUND MicroRNAs (miRNAs) are ubiquitous non-coding RNAs that regulate gene expression at the post-transcriptional level. An increasing number of studies has revealed that viruses can also encode miRNAs, which are proposed to be involved in viral replication and persistence, cell-mediated antiviral immune response, angiogenesis, and cell cycle regulation. Singapore grouper iridovirus (SGIV)...