Low molecular weight organic acids are most predominant in apple juice among these components fumaric acid was not exceed more than 5 ppm. It was thought that the HPLC is the best method of the determination of organic acids in the apple juice. In this study, chromatographic separation of organic acids of apple juice was obtained by preparing a sample and by applying them to acid phase for ...

We have performed a detailed characterization and identification of wheat gluten proteins obtained from the Teal variety of Canadian hard red spring wheat. RP-HPLC separation of the sample into 35 fractions has reduced the spectral complexity; this was followed by MALDI mass spectrometry (MS), which showed the presence of six or fewer resolved protein components above 20 kDa in each RP-HPLC fra...

Purpose: To describe a chemoenzymatic approach joining an enzymatic regioselective hydrolysis of peracetylated N-acetyl-α-D-glucosamine (A) with a mild controlled acyl relocation which resulted 2-acetamido-2 deoxy-1,3,6-tri-O-acetyl-α-D-glucopyranose (1B). Methods: Immobilization of lipase on decaoctyl (DSEOD) and octyl-agarose (OSCL) was carried out as reported by the work of Bastida et al. Th...

A simple, precise and rapid RP-HPLC method was developed for the determination of ebastine in pharmaceutical formulations. The method was carried out on a Phenomenex RP-C18 column using a mixture of methanol and water (90:10) and detection was done at 262 nm. The linearity range was 5-100 mug/ml. The intra-day and inter-day precision were in the range of 0.22% to 0.49% and 0.24% to 0.73%, respe...

Background. Peptides are important components of foods mainly due to their biological activity. The basic method of their identification is reversed phase high-performance liquid chromatography coupled with electrospray-ionization mass spectrometry (RP-HPLC-ESI-MS). Retention time (tR) prediction in silico is very helpful in analysis of multicomponent peptide mixtures. One of problems associate...

Anthocyanins, flavonoid compounds present in grapes and wines, were determined by reverse-phase high-performance liquid chromatography (RP-HPLC) with electrochemical detection (RP-HPLC-ED). The method developed consists of RP-HPLC gradient elution with voltammetric detection using a glassy carbon electrode after separation in an Inertsil ODS-3V analytical column. Good peak resolution was obtain...

To establish an RP-HPLC method for the determination of β-sitosterol content in Fructus Bruceae extract; and to observe its inhibitory effect on gastric cancer metastasis. β-sitosterol content in Fructus Bruceae extract was determined by RP-HPLC. Inhibitory effects of low-, mediumand high-dose extracts on the adhesion of human gastric cancer SGC-7910 cells to Fibronectin (FN) were detected by M...

We report the first description studies, partial characterization, and intraspecific difference of Centruroides edwardsii, Gervais 1843, venom. C. edwardsii from two Colombian regions (Antioquia and Tolima) were evaluated. Both venoms showed hemolytic activity, possibly dependent of enzymatic active phospholipases, and neither coagulant nor proteolytic activities were observed. Venom electropho...

Theobromine and its related compounds, such as caffeine and theophylline, are secondary metabolites that belong to the alkaloids and have economic and cultural importance. These alkaloids have demonstrated stimulatory effects on the central nervous, gastrointestinal, cardiovascular, renal and respiratory systems, resulting in 'energy arousal', increased motivation to work, increased alertness a...

Lipophilicity, expressed by log P, is an important physicochemical property of drugs that affects many biological processes, including drug absorption and distribution. The main purpose of this study to determine the log P values of newly discovered drug leads using reversed-phase high-performance liquid chromatography (RP-HPLC). The reference standards, with varying polarity ranges, were disso...