To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions...

Geoduck clams (Panopea generosa) are an increasingly important fishery and aquaculture product along the eastern Pacific coast from Baja California, Mexico, to Alaska. These long-lived clams are highly fecund, although sustainable hatchery production of genetically diverse larvae is hindered by the lack of sexual dimorphism, resulting in asynchronous spawning of broodstock, unequal sex ratios, ...

Coordination of animal behavior with reproductive status is often achieved through elaboration of hormones by the gonad. In the nematode Caenorhabditis elegans, adult males explore their environment to locate mates. Mate searching is regulated by presence of mates, nutritional status, and a signal from the gonad. Here we show that the gonadal signal acts via the nuclear receptor DAF-12, a prote...

During development, cells divide, differentiate into various cell types, and organize into functional tissues and organs. We are interested in understanding the cellular and molecular basis of organ formation in the nematode Caenorhabditis elegans. To this end, we are investigating two novel proteins that have a role in gonadogenesis, SYS-1 and NUD-1. SYS-1 was identified via a genetic screen f...

The mammalian sex-determining pathway is controlled by the presence or absence of SRY expression in the embryonic gonad. Expression of SRY in males is believed to initiate a pathway of gene expression resulting in testis development. In the absence of SRY, ovary development ensues. Several genes have now been placed in this pathway but our understanding of it is far from complete and several fu...

The Caenorhabditis elegans teneurin ortholog, ten-1, plays an important role in gonad and pharynx development. We found that lack of TEN-1 does not affect germline proliferation but leads to local basement membrane deficiency and early gonad disruption. Teneurin is expressed in the somatic precursor cells of the gonad that appear to be crucial for gonad epithelialization and basement membrane i...

During mouse development, the gonad begins to form shortly before 10. 5 days postcoitum (dpc) on the ventromedial side of the mesonephros. The XY gonad consists of germ cells and somatic cells. The origin of the germ cells is clearly established; however, the origin of the somatic cells, especially the epithelial supporting cell lineages, called Sertoli cells, is still unclear. Sertoli cells ar...

Vestimentiferan tubeworms are significant members of deep-sea chemosynthetically-driven communities, including hydrothermal vents and cold seeps. The reproductive condition in this taxon is rarely studied because of sampling constraints inherent to these environments, and the lack of a simple methodology to quantify gonad development in vestimentiferans. We demonstrate that the amount of gonad ...

In many animals, germ cells migrate to the gonad to assemble into a functional organ. Recent work in Drosophila has built a picture of the gene activities that specify the gonad and allow it to attract germ cells, and has led to the identification of a gene, columbus, that may encode the attractive factor.

Abstract Kawakawa (Euthynnus affinis) is one of the economically valuable species for Mallaca strait fishermen. This study aims to determine level gonad maturity, size fish’s first ripe gonad, and maturity index (E. caught at Tanjung Beringin’s Auction Place, Serdang Bedagai Regency, North Sumatra. Three months sampling were conducted, from July September 2021. The maturation gonads was observe...