abstractbackground : in order to quantify effects of ultrasound irradiation parameters under therapeutic condition, especially sonodynamic therapy, it is initially necessary to evaluate inertial cavitation activity in vitro conditions therefore, in this study, the effect of 1 mhz low level ultrasound based on °oh radicals generated by acoustic inertial cavitation in aqueous solution was monitor...

The use of power ultrasound refers to the application of sound that is just above the range of human hearing. Under these conditions, an acoustic wave is able to create very high levels of turbulence within an aqueous solution, through the generation of transient cavitation, the growth and violent collapse of microbubbles. Many scientists will be aware of the use of ultrasonic cell disruptors a...

From the viewpoint of safety, non-viral vector systems represent an attractive gene delivery system for gene therapy. However, the transfection efficiency of non-viral vectors in vivo is generally very low. Previously, it was reported that microbubbles, utilized as imaging agents for diagnostic echocardiography, could promote gene delivery into cells when combined with ultrasound exposure. We t...

Sonoporation is the membrane disruption generated by ultrasound and has been exploited as a non-viral strategy for drug and gene delivery. Acoustic cavitation of microbubbles has been recognized to play an important role in sonoporation. However, due to the lack of adequate techniques for precise control of cavitation activities and real-time assessment of the resulting sub-micron process of so...

Amongst the variety of complex phenomena encountered in nonlinear physics, a hysteretic effect can be expected on ultrasound cavitation due to the intrinsic nonlinearity of bubble dynamics. When applying successive ultrasound shots for increasing and decreasing acoustic intensities, a hysteretic behaviour is experimentally observed on inertial cavitation activity, with a loop area sensitive to ...

BACKGROUND We have previously shown that continuous-wave ultrasound can rapidly dissolve human thrombi in vitro, with 99% of all residual particles measuring less than 10 microns in diameter. To assess the effects of pulsed-wave ultrasound energy on whole blood clots, 1) in vitro studies were preformed to assess precisely the rates of clot disruption and to quantify particulate size, and 2) in ...

We introduce a Cavitation Intensifying Bag as a versatile tool for acoustic cavitation control. The cavitation activity is spatially controlled by the modification of the inner surface of the bag with patterned pits of microscopic dimensions. We report on different measurements such as the transmission of ultrasound, temperature increase inside the bag during sonication. Several applications of...

Using conditions different from conventional medical imaging or laboratory cell lysis, ultrasound has recently been shown to reversibly increase plasma membrane permeability to drugs, proteins and DNA in living cells and animals independently of cell or drug type, suggesting a ubiquitous mechanism of action. To determine the mechanism of these effects, we examined cells exposed to ultrasound by...

This paper discusses various interactions between ultrasound, phospholipid monolayer-coated gas bubbles, phospholipid bilayer vesicles, and cells. The paper begins with a review of microbubble physics models, developed to describe microbubble dynamic behavior in the presence of ultrasound, and follows this with a discussion of how such models can be used to predict inertial cavitation profiles....

In animal experiments, the pathogenesis of lung hemorrhage due to exposure to clinical diagnostic levels of ultrasound has been attributed to an inertial cavitation mechanism. The purpose of this article is to report the results of two experiments that directly contradict the hypothesis that ultrasound-induced lung hemorrhage is caused by inertial cavitation. Elevated hydrostatic pressure was u...