The irreversible formation of stable glycosylated hemoglobin proceeds through a labile intermediate that is indistinguishable, by most methods, from the stable glycosylated product. The inclusion of the labile intermediate, which changes acutely with acute blood glucose changes, detracts from the utility of the assay as an index of chronic glucose concentration. We have developed a rapid, relia...

A boronate affinity column method for the measurement of glycosylated hemoglobins was evaluated. In the procedure the glycosylated hemoglobins were bound by immobilized boronic acid to separate them from nonglycosylated hemoglobins. Elution of bound glycosylated hemoglobins was carried out with sorbitol buffer, and the absorbance was read at 414 nm. The method was linear to a glycosylated hemog...

We present data on the use of filter-paper blood collection for measurement of glycosylated whole-blood proteins (gWB) (hemoglobin and plasma proteins). A capillary blood sample, obtained by fingerprick, is spotted directly onto filter paper (Schleicher & Schuell 903). The blood spot is washed briefly with alcohol (ethanol or isopropanol) to remove free glucose and dried before shipment to the ...

The features of the vasoregulating function vascular endothelium have been determined and correlations between haemodynamic metabolic indices established in patients with coronary heart disease associated type II diabetes mellitus. In mellitus comparison without an association significantly higher content blood fibrinogen diameter brachial artery (after decompression) were observed. Direct fast...

Our aim was to evaluate oxidative stress in diabetes mellitus by the measurement of biophysical parameters (changes) of hemoglobin macromolecule and some biochemical parameters on two groups of diabetic patients, non-insulin dependent diabetes mellitus (NIDDM), and insulindependent diabetes mellitus (IDDM). This study has been conducted on 45 NIDDM, 30 IDDM, compared to 20 healthy subjects. Blo...

BACKGROUND While diabetes mellitus (DM) is a known risk factor for tuberculosis, the prevalence among TB patients in India is unknown. Routine screening of TB patients for DM may be an opportunity for its early diagnosis and improved management and might improve TB treatment outcomes. We conducted a cross-sectional survey of TB patients registered from June-July 2011 in the state of Kerala, Ind...

BACKGROUND Glycosylated hemoglobin (HbA1c) in diabetic patients reflects the average blood glucose level, and will not be affected by variability in blood glucose in short time. Regular care of patients by medical staff could effectively control glycemic situation. The aim of this study was to assess the effect of medical care by general physicians on glycemic control by measuring of HbA1c. M...

Accepted October 13, 1983 It is well known that glycosylated hemo globin (HbA1) increases in the blood of diabetic patients. However, only few studies have been reported on the changes in the values of HbA1 in mice and rats. The degree of increase in HbA1 levels was not correlated with the severity of hyperglycemia, duration of diabetes, age of the mouse, or body weight in C57BL/KsJ mice (1). I...

OBJECTIVES To measure the efficacy of new insulin analogues compared with the conventional types of insulin, and to compare their effects on patient satisfaction regarding their weight changes and the frequency of hypoglycemic episodes. METHODS In this retrospective cohort observational study, data was collected from the medical records of 122 eligible diabetics on insulin therapy attending g...