Multidrug resistance (MDR) remains a major obstacle to successful chemotherapeutic treatment of cancer and can be caused by overexpression of P-glycoprotein, the MDR1 gene product. To further validate a knockdown approach for circumventing MDR, we developed a P-glycoprotein inhibition strategy using short hairpin RNA interference (shRNAi) and now show efficacy and target specificity in vivo. Tw...

background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...

the main aim of this study was to obtain the crimean-congo hemorrhagic fever virus (cchfv) glycoprotein, through either stable transgenic plants or using a transient expression system, and determine the yield, quality and finally the immunogenicity of the plant-made cchfv glycoprotein in a mouse model. we designed and synthesized a codon-optimized g1/g2 gene from the g1 and g2 parts of the cchf...

Lyssaviruses are unsegmented RNA viruses causing rabies. Their vectors belong to the Carnivora and Chiroptera orders. We studied 36 carnivoran and 17 chiropteran lyssaviruses representing the main genotypes and variants. We compared their genes encoding the surface glycoprotein, which is responsible for receptor recognition and membrane fusion. The glycoprotein is the main protecting antigen an...

brain capillaries and crossreactivity of one antibody with a muscle protein. J Histochem Cytochem 1989;37:159–64. (34) Beaulieu E, Demeule M, Pouliot JF, Averill-Bates DA, Murphy GF, Beliveau R. P-glycoprotein of blood brain barrier: cross-reactivity of Mab C219 with a 190 kDa protein in bovine and rat isolated brain capillaries. Biochim Biophys Acta 1995;1233:27–32. (35) Rao VV, Anthony DC, Pi...

P-glycoprotein is a plasma membrane protein believed to mediate resistance to natural product drugs such as vincristine, Adriamycin, and actinomycin D. To facilitate the study of human P-glycoprotein, monoclonal antibodies (designated HYB-612, HYB-241, and HYB-195) were raised against vincristine-resistant human neuroblastoma (SH-SY5Y/VCR) cells. The antibodies recognize a Mr 180,000 plasma mem...

Ascorbic acid (AsA) or Vitamin C is an antioxidant molecule and plays important role in many biological processes plants. GDP-D-mannose pyrophosphorylase (GMP VTC1) catalyzes the synthesis of GDP-D-mannose, which a precursor for AsA production used cell wall polysaccharide glycoprotein synthesis. In rice, OsVTC1 gene consists three homologs, including OsVTC1-1, OsVTC1-3 OsVTC1-8. this study, we...