OBJECTIVE The purpose of this study was to investigate the influence of AMP-activated protein kinase (AMPK) activation on protein acetylation and glycolysis in postmortem muscle to better understand the mechanism by which AMPK regulates postmortem glycolysis and meat quality. METHODS A total of 32 mice were randomly assigned to four groups and intraperitoneally injected with 5-Aminoimidazole-...

The decrease in plasma lactate during dichloroacetate (DCA) treatment is attributed to stimulation of lactate oxidation. To determine whether DCA also inhibits lactate production, we measured glucose metabolism in muscles of fed and fasted rats incubated with DCA and insulin. DCA increased glucose-6-phosphate, an allosteric modifier of glycogen synthase, approximately 50% and increased muscle g...

Cancer cells prefer aerobic glycolysis, but little is known about the underlying mechanism. Recent studies showed that the rate-limiting glycolytic enzymes, pyruvate kinase M2 (PKM2) directly phosphorylates H3 at threonine 11 (H3T11) to regulate gene expression and cell proliferation, revealing its non-metabolic functions in connecting glycolysis and histone modifications. We have reported that...

Cells need to adapt to dynamic environments. Yeast that fail to cope with dynamic changes in the abundance of glucose can undergo growth arrest. We show that this failure is caused by imbalanced reactions in glycolysis, the essential pathway in energy metabolism in most organisms. The imbalance arises largely from the fundamental design of glycolysis, making this state of glycolysis a generic r...

An increased flux through glycolysis supports the proliferation of cancer cells by providing additional energy in the form of ATP as well as glucose-derived metabolic intermediates for nucleotide, lipid, and protein biosynthesis. Thus, glycolysis and other metabolic pathways that control cell proliferation may represent valuable targets for therapeutic interventions and diagnostic procedures. I...

mutagenesis to replace all the wild-type tryptophan residues of the protein, usually by tyrosine. (ii) Measurement of the properties of the tryptophan-less mutant to establish these have not been changed by mutation; (iii) Insertion of a single tryptophan residue a t the site where motion is to be measured. (iv) Characterization of that motion from the changed single-tryptophan fluorescence int...

The series of experiments presented in this paper demonstrate certain relationships in the progressive changes of sugar and phosphorus observed in defibrinated blood incubated in vitro at 370 C. through periods of from ten to twenty-four hours. The accompanying figures illustrate a pattern of these changes which may be regarded as characteristic of normal bloods and bloods of normal animals ren...

In vertebrates, retinal rod and cone photoreceptor cells rely significantly on glycolysis. Lactate released from fuels neighboring pigment epithelium Müller glial through oxidative phosphorylation. To understand this highly heterogeneous metabolic environment around cells, single-cell analysis is needed. Here, we visualized cellular AMP-activated protein kinase (AMPK) activity ATP levels in the...

When blood undergoes glycolysis at 37 ~ C, in addition to the loss of blood sugar, there are accompanying changes in the inorganic phosphorus. This relationship has been investigated and reported by a number of authors (2, 8, 9, 10). They agree that during the first 4 hours of glycolysis, the inorganic phosphorus of the blood in normal individuals either remains at a constant level, or is only ...