A number of unusual and rare carbohydrates were tested as potential inhibitors of various glycosidases, as well as inhibitors of N-linked oligosaccharide processing. The best inhibitors of several arylglycosidases and of glucosidase I were L-xylulose and L-fructose. Both of these sugars showed some inhibitory activity towards yeast alpha-glucosidase but were inactive against beta-glucosidase an...

Glycosidases are the enzymes involved in various biochemical processes related to metabolic disorders and diseases. Therefore, much effort has been focused on searching novel pharmacotherapy for the treatment of these ailments from medicinal plants due to higher safety margins. To pursue these efforts, the present study was performed to evaluate the α-glucosidase inhibitory activities of thirty...

Purpose: To isolate and evaluate α-glucosidase inhibitors from dichloromethane extract of Croton bonplandianum Baill as probable remedy for management of diabetes. Methods: Activity-guided isolation of constituents from dichloromethane extract was carried out. Fractionation of dichloromethane extract by column chromatography on silica gel and Sephadex LH 20 using different mobile phase systems ...

The design of covalent inhibitors in glycoscience research is important for the development of chemical biology probes. Here we report the synthesis of a new carbocyclic mechanism-based covalent inhibitor of an α-glucosidase. The enzyme efficiently catalyzes its alkylation via either an allylic cation or a cationic transition state. We show this allylic covalent inhibitor has different catalyti...

Miglitol is an alpha-glucosidase inhibitor that improves post-prandial hyperglycemia, and it is the only drug in its class that enters the bloodstream. Anecdotally, miglitol lowers patient body weight more effectively than other alpha-glucosidase inhibitors, but the precise mechanism has not been addressed. Therefore, we analyzed the anti-obesity effects of miglitol in mice and in the HB2 brown...

Postprandial hyperglycemia is a predisposing factor for vascular dysfunction and organ damage. α-glucosidase is a hydrolytic enzyme that increases the glucose absorption rate and subsequently elevates blood glucose levels. Garlic (Allium sativum L.) is a rich source of several phytonutrients, including thiosulfinate (THIO). The aim of this study was to evaluate the ability of THIO, a potent inh...

Cladonia foliacea (CF) is a type of lichen belonging to the Cladoniaceae family, used as traditional medicine for various diseases. It known that CF has strong antioxidant and antidiabetic effects originating from secondary components. However, bioactivity significantly affected by extraction conditions such temperature, liquid/solid ratio, solvent type/concentration. Herein, parameters (temper...

elm leaf beetle, xanthogaleruca luteola, is a serious pest (particularly at its larval stage) of elm trees in many areas. the existence of three important enzyms namely: ?-amylase, ?- glucosidase and ?-glucosidase in the midgut of the insect at its larval stage was investigated. optimum activity for ?-, ?-glucosidase and ?-amylase was recorded at phs 5.0, 6.0 and 6.0 against p-nitrophenyl-?-d-g...

BACKGROUND Some Ficus species have been used in traditional African medicine in the treatment of diabetes. The antidiabetic potential of certain species has been confirmed in vivo but the mechanism of activity remains uncertain. The aim of this study was to determine the activity and to investigate the mechanism of antidiabetic activity of ten selected Ficus species through inhibition of α-amyl...

The periodic rise and frequency of diabetes mellitus (DM) have pressured into the quest for other possible anti-diabetic remedies from medicinal plants. foremost strategies engaged in lessening gastrointestinal glucose levels by hindrance carbohydrate digesting enzyme prominently as α-glucosidase . Acarbose, currently used an inhibitor, is a useful treatment option management hyperglycemia type...