Most biological fluids contain both neutral and acid alpha-glucosidase. Optimal conditions were therefore developed for the selective determination of the activity of neutral and acid alpha-glucosidase, using 2-step, discontinuous assays. In the first step of the assay of neutral alpha-glucosidase, glucose was liberated from maltose (citrate-phosphate buffer, pH 6.8, 20 mmol/l maltose, 25 mmol/...

Phytochemical study of the roots Erythrina senegalensis led to isolation a new α-sophoradiol glycoside, erythrinoside (1), together with four known compounds, lupeol (2), (3), isoneorautenol (4) and D-mannitol (5). The structures compounds were elucidated using spectroscopic data including 1D 2D NMR, mass spectrometry by comparison made some reported previously; samples (extracts compounds) als...

This study aims at exploring earthworm protein hydrolysate and its peptide fractions as a potential hypoglycemic agent by inhibiting α-amylase α-glucosidase. Firstly, the best hydrolysis conditions to gain hydrolysates with highest inhibitory activity (α-AIA) α-glucosidase (α-GIA) were figured out using one-factor test. Next, stability of their 5 recovered ultrafiltration membranes assessed emp...

2-Aminoresorcinol is a potent and selective intestinal glucosidase inhibitor. Unlike the majority of glucosidase inhibitors, it shows an uncompetitive mode of inhibition. In this study, we tested the intestinal glucosidase inhibitory activity of various 2-aminoresorcinol derivatives. We found that structural changes, in amino and two phenolic hydroxyl groups had a negative impact on inhibitory ...

Gaucher disease type 1, a non-neuronopathic lysosomal storage disease, is caused by mutations at the acid beta-glucosidase locus. Periodic infusions of macrophage-targeted acid beta-glucosidase reverse hepatosplenomegaly, hematologic, and bony findings in many patients. Two patients receiving enzyme therapy developed neutralizing antibodies to acid beta-glucosidase that were associated with a l...

morphogenesis and development of fungal organisms 3. Kirk, T. K. ( 1983) in ihe Fi/urnmiotc.$ Ftrn,qi (Smith, J . E.. Berry, I 10. 1 1 I . This could be one of the reasons for the Dresence of D. R. & Kristiansen. B.. eds.), vol. 4, pp. 265-295, Edward ;he' fl-ilucosidase during the first period of bioiynthesis. It has also been proposed that fl-glucosidase may be important for glycoprotein synt...

In our continued investigations of the plant Caryopteris incana, five new iridoid glucosides 1-5, including two cis-trans-isomers, 3 and 4, along with six known compounds 6-11, were isolated from the n-butyl alcohol (n-BuOH) soluble fraction of whole dried material of Caryopteris incana. Their structures were established by a combination of spectroscopic techniques, including 1D and 2D NMR and ...

bilberry [vaccinium myrtillus linn. (ericaceae)] fruit (vmf) has been traditionally used for treatment of bladder stones, biliary disorders, scurvy, coughs, and lung tuberculosis. vmf may have some pharmaceutical properties owing to these uses, but in vivo and in vitro studies are limited for clarification of medicinal activity and its antidiabetic and antioxidant properties are not investigate...

BACKGROUND A complete saccharification of plant polymers is the critical step in the efficient production of bio-alcohols. Beta-glucosidases acting in the degradation of intermediate gluco-oligosaccharides produced by cellulases limit the yield of the final product. RESULTS In the present work, we have identified and then successfully cloned, expressed, purified and characterised 4 highly act...

There was an ionic interaction between acidic polysaccharides (APS) and proteins at the pH range in which APS were negatively charged and proteins were positively charged, and in enzymes the interaction was detected as a change in the enzyme activity. At pH 4.7, acid phosphatase (pI, 5.4), alpha-glucosidase (pI, 5.7), and beta-glucosidase (pI, 7.3) were inhibited by APS to various extents. On t...