To the Editor: Hand-held glucose meters are a practical solution for measuring glucose in neonatal intensive care units and special care nurseries because of their portability, the immediacy of results, and minimal blood volume requirements compared with dispatching samples for central laboratory analysis. We have used the Roche Accu-chek Advantage II meter for some time in our neonatal intensi...

Rabbit liver UDP-glucose pyrophosphorylase has been crystallized and further purified to a specific activity of 200 by using preparative centrifugation with sucrose density gradients. The pyrophosphorylase requires a divalent cation. Magnesium is preferred, although manganese, cobalt, and calcium can serve as less effective alternate cation activators. Maximum activity requires a reducing agent...

Lactose and neuramin lactose were produced by incubating subcellular particles from rat mammary gland in a system containing glucose, uridine diphosphate galactose, and cytidine monophosphate N-acetylneuraminic acid. The K, values for glucose in the synthesis of neuramin lactose were fairly constant for three similarly prepared enzyme preparations (0.25, 0.17, and 0.22 m&r), but were widely dis...

When Lactococcus lactis subsp. lactis IL1403 or L. lactis subsp. cremoris MG1363 is grown in a medium with galactose as the carbon source, the culture lyses to a lesser extent in stationary phase than when the bacteria are grown in a medium containing glucose. Expression of AcmA, the major autolysin of L. lactis, is not influenced by the carbon source. Binding studies with a fusion protein cons...

[1] We show that spherical harmonic (SH) solutions of the Gravity Recovery and Climate Experiment (GRACE) are now of sufficient quality to observe effects of co-seismic and post-seismic deformation due to the rupture from the Mw = 9.3 Sumatra-Andaman earthquake on December 26, 2004, and its companion Nias earthquake (Mw = 8.7) on March 28, 2005. The improved GGM 03 SH (Level 2) solutions, and i...

BACKGROUND In Saccharomyces cerevisiae galactose is initially metabolized through the Leloir pathway after which glucose 6-phosphate enters glycolysis. Galactose is controlled both by glucose repression and by galactose induction. The gene PGM2 encodes the last enzyme of the Leloir pathway, phosphoglucomutase 2 (Pgm2p), which catalyses the reversible conversion of glucose 1-phosphate to glucose...

A highly stereoselective, efficient and facile route was achieved for the synthesis of novel biochemically potent sugar fused pyrano[3,2- c ]pyranone derivatives starting from inexpensive, naturally occurring d -galactose -glucose.

Lactase-phlorizin hydrolase (LCT), more commonly known as lactase, is an enzyme responsible for cleaving lactose into absorbable monosaccharides, glucose and galactose. LCT deficiency (hypolactasia – HL) is caused by a decreased activity of LCT in the small intestinal villi and potentially results in lactose malabsorption what may lead to the development of clinical symptoms (diarrhea, bloating...

PURPOSE There is increasing interest into the potentially beneficial effects of galactose for obesity and type 2 diabetes management as it is a low-glycemic sugar reported to increase satiety and fat mobilization. However, fructose is also a low-glycemic sugar but with greater blood pressure elevation effects than after glucose ingestion. Therefore, we investigated here the extent to which the ...

Lactose and neuramin lactose were produced by incubating subcellular particles from rat mammary gland in a system containing glucose, uridine diphosphate galactose, and cytidine monophosphate N-acetylneuraminic acid. The K, values for glucose in the synthesis of neuramin lactose were fairly constant for three similarly prepared enzyme preparations (0.25, 0.17, and 0.22 m&r), but were widely dis...