strychnos henningsii gilg is recommended among other remedies for the treatment of diabetes in traditional medicine of southern africa. the antidiabetic effect of oral administration of aqueous bark extract of the plant at 125, 250 and 500 mg/kg body weight was investigated in diabetic rats induced with streptozotocin-nicotinamide for 15 days. the extract decreased the blood glucose level, feed...

In this work we explore the micro-ring resonator platform to study the diffusion-driven mass transport of small molecules within microfluidic channels. The micro-ring resonators are integrated on a silicon-on-insulator photonic chip and combined with microfluidics in poly(dimethylsiloxane) (PDMS). We apply a strong initial gradient in the solute concentration and use the micro-ring resonators t...

The ability to create biocompatible well-controlled membranes has been an area of great interest over the last few years, particularly for biosensor applications. The present study describes the fabrication and characterization of novel nanoporous micromachined membranes that exhibit selective permeability and low biofouling. Results indicate that such membranes can be fabricated with uniform p...

BACKGROUND AND PURPOSE The pathophysiology of hypoglycemia shares a common mechanism with cerebral ischemia, but so far, little is known regarding MRI of humans with hypoglycemia. METHODS We report a patient with left hemiparesis and dysarthria associated with a blood glucose level of 1.7 mmol/L. The patient recovered completely after glucose infusion. RESULTS The initial diffusion-weighted...

Moderate concentrations of ethanol reduce the velocity of uptake of three representative Na+-symport systems (D-glucose, L-alanine, L-ascorbate), whether electrogenic (the first two) or electroneutral (L-ascorbate). This 'inhibition' is observed only if these transport systems are tested in the presence of an initial Na+ gradient (out greater than in); no inhibition is found in tracer-equilibri...

A simple gel immunodiffusion agar procedure was developed for detecting toxigenic strains of Clostridium botulinum type A. The method consisted of overlaying colonies grown on thin-layer tryptone-peptone-glucose-yeast extract agar with gel diffusion agar containing desired levels of C. botulinum type A antitoxin. Concentric precipitin zones formed around colonies of C. botulinum type A. Strains...

Uptake of labeled Dand L-glucose has been shown to occur with highly purified brush border membranes from the epithelial cells of rat small intestine using a Millipore filtration technique. An intact glucose carrier system in the isolated membranes was demonstrated as evidenced by the following. (a) D-Glucose was taken up and released faster than L-glucose. (b) Sodium ions increased initial rat...

BACKGROUND AND PURPOSE Impaired cortical venous outflow and abnormal deep venous collaterals are common in Sturge-Weber syndrome (SWS), but their relation to brain metabolism and function is poorly understood. In this study, advanced MR imaging techniques, such as susceptibility-weighted imaging (SWI) and diffusion tensor imaging (DTI), were applied in conjunction with positron-emission tomogra...

Uridine diphosphate glucose pyrophosphorylase (EC 2.7.7.9) from Dictyostelium discoideum has been purified to apparent physical and immunochemical homogeneity. The molecular weight estimates from gel diffusion and equilibrium sedimentation analyses are 390,000 and 384,000, respectively. The enzyme is polymeric apparently composed of a single monomeric species with a molecular weight of 55,000 a...

A fractal analysis is used to model the binding and dissociation kinetics of connective tissue interstitial glucose, adipose tissue interstitial glucose, insulin, and other related analytes on biosensor surfaces. The analysis provides insights into diffusion-limited analyte-receptor reactions occurring on heterogeneous biosensor surfaces. Numerical values obtained for the binding and the dissoc...