Antianemic medicament ferrous gluconate, ferrous fumarate, and a Dynabi tablet with a basic iron bearing ingredient were studied with the use of Mössbauer spectroscopy. Room temperature spectra of ferrous gluconate gave clear evidence that the two phases of iron were present: ferrous (Fe2+) as a major one with a contribution at and above 91 a.u.% and ferric (Fe3+) whose contribution was found t...

Here we report functional characterization of the Streptomyces coelicolor M145 gene SCO1678, which encodes a GntR-like regulator of the FadR subfamily. Bioinformatic analysis suggested that SCO1678 is part of putative operon (gnt) involved in gluconate metabolism. Combining the results of SCO1678 knockout, transcriptional analysis of gnt operon, and Sco1678 protein-DNA electromobility shift ass...

Induction of Entner-Doudoroff pathway enzymes in Pseudomonas fluorescens was investigated to study the role of gluconate as a possible inducer. Glucose oxidase-deficient mutants were isolated and characterized. One of these mutants, gox-7, was deficient in particulate glucose oxidase; another mutant, gox-17, was deficient in particulate glucose and gluconate oxidase activities. Gluconate, but n...

The aim of this study was to compare the antibacterial activities of Er,Cr:YSGG laser with two different power outputs against a chlorhexidine gluconate-based cavity disinfectant. A cavity tooth model test was used to determine the antibacterial activity. Four cylindrical cavities were prepared on the dentin surface of 10 bovine incisors and left in contact with Streptococcus mutans for 72 hour...

INTRODUCTION Several studies have evaluated the efficacy of home use of chlorhexidine before surgery to reduce bacterial colonization. However, these studies have provided conflicting evidence about the potential efficacy of this strategy in decreasing bacterial loads and infection rates across surgical populations, and no prior study has analyzed the benefit of this intervention before spine s...

The gntT gene of Escherichia coli is specifically induced by gluconate and repressed via catabolite repression. Thus, gluconate is both an inducer and a repressor of gntT expression since gluconate is a catabolite-repressing sugar. In a gntR deletion mutant, the expression of a chromosomal gntT::lacZ fusion is both high and constitutive, confirming that GntR is the negative regulator of gntT. I...

The fate of nutrients during storage of longissimus dorsi muscle at 4 degrees C was examined. Glucose concentrations in meat were shown to decrease concomitantly with an approximately fourfold increase in the activity of glucose dehydrogenase. Gluconate concentrations in meat were determined by an enzyme assay and shown to increase from 2.1 to 40.6 microgram/g upon storage of the meat from day ...

Four hundred and eighty mixed-sex broiler chicks aged 3 h after hatching were allotted according to a completely random design in a 6 × 2 × 2 factorial schedule into two groups of 12 replications of 20 chicks each. The main experimental factors were fasting for 0, 6, 12, 24, 36 and 48 h after chick placement and calcium gluconate (Ca-glu) injection (0 and 0.6 ml). Live body weight (BW) of chick...

The aim of this study was to evaluate the efficacy of zinc gluconate, either associated or not to dimethyl sulfoxide (DMSO), as a contraceptive method for the canine male population. Twenty-nine sexually mature male dogs were divided into five groups: Group I (control, saline solution); Group II (zinc gluconate 13.1 mg); Group III (zinc gluconate 26.2 mg); Group IV (zinc gluconate 13.1 mg and D...

Cut muscle fibers from Rana temporaria (sarcomere length, 3.5-3.9 micro(m); 14-16 degreesC) were mounted in a double Vaseline-gap chamber and equilibrated with an external solution that contained tetraethyl ammonium- gluconate and an internal solution that contained Cs as the principal cation, 20 mM EGTA, and 0 Ca. Fibers were stimulated with a voltage-clamp pulse protocol that consisted of pul...