BACKGROUND Type 2 diabetes is a serious problem for developed countries. Prevention of prediabetes progression to type 2 diabetes with the use of natural products appears to a cost-effective solution. Previously we showed that enzymatically digested low molecular weight chitosan-oligosaccharide with molecular weight (MW) below 1,000 Da (GO2KA1) has potential for hyperglycemia management. METH...

The rate of a reaction catalyzed by enzymes is influenced by various factors, including temperature, pH, chemical substances, etc.1 In this work, the influence of temperature on enzyme operational stability was studied. Temperature deactivation of an enzyme is usually defined as a transformation of an active enzyme into denaturated (deactivated) form, described by an irreversible first-order re...

Abstract Insulin concentrations are variable in bovine colostrum (5 to 263 µg insulin/L) and its effects on gastrointestinal function neonatal calves unknown. The objective of this study was determine if varying insulin would influence intestinal carbohydrases Holstein bulls. Calves (n = 26; 49.11 kg, SD 3.24) were removed from their dams after calving. They fed 3 meals (7% BW or 3.10 ± 0.02 L)...

A recombinant plasmid pool of the Saccharomyces diastaticus genome was constructed in plasmid YEp13 and used to transform a strain of Saccharomyces cerevisiae. Six transformants were obtained which expressed amylolytic activity. The plasmids each contained a 3.9-kilobase (kb) BamHI fragment, and all of these fragments were cloned in the same orientations and had identical restriction maps, whic...