We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of...

BACKGROUND AND AIMS Early detection of fibrosis is important in identifying individuals at risk for advanced liver disease in non-alcoholic fatty liver disease (NAFLD). We tested whether second-harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) microscopy, detecting fibrillar collagen and fat in a label-free manner, might allow automated and sensitive quantification of e...

We investigate second harmonic generation (SHG) from a photonic crystal slab consisting of centrosymmetric materials. The SHG signal is observed in the transmission direction when the incident laser excites the quasiwaveguide mode. As the SHG frequency approaches the exciton level, the SHG intensity increases resonantly. When the incident angle is exactly 0, the SHG signal vanishes even if the ...

Although second harmonic generation (SHG) microscopy provides unique imaging advantages for voltage imaging and other biological applications, genetically-encoded SHG chromophores remain relatively unexplored. SHG only arises from non-centrosymmetric media, so an anisotropic arrangement of chromophores is essential to provide strong SHG signals. Here, inspired by the mechanism by which K-Ras4B ...

The aim of this work was to study the sarcomeric organization of muscles with PSE-like zones and normal muscle (control), focusing on the SHG signal pattern of myosin (single or double band). Second harmonic generation microscopy has been applied on pork Semimembranosus samples fixed at 24 hours post mortem. Electronic microscopy was applied on the same samples to improve SHG images interpretat...

The macromolecular structure of purified cellulose samples is studied by second-harmonic generation (SHG) imaging microscopy. We show that the SHG contrast in both Valonia and Acetobacter cellulose strongly resembles that of collagen from animal tissues, both in terms of morphology and polarization anisotropy. Polarization analysis shows that microfibrils in each lamella are highly aligned and ...

Second harmonic generation (SHG) response is one of the most essential properties of nonlinear optical (NLO) materials. To achieve relatively strong SHG response, a feasible strategy is to introduce stereochemically active lone pair (SCALP) cations, such as Bi3+. NLO materials with Bi3+ usually exhibit a huge difference in SHG response, the inner correlation between the activity of SCALP and SH...

In situ optical second harmonic generation (SHG) technique was employed to investigate the shape and density of Cu nanoclusters, which were electrochemically formed on p-GaAs(001) electrode surfaces. Since GaAs is not a centrosymmetric medium, a significant portion of SHG signal arises from the bulk dipole susceptibility, but it was possible to separate a surface-induced signal from a bulk-indu...

We investigate if participation in Indian Self Help Group microfinance program (SHG) results in reducing vulnerability. Using theSHG rural household survey data collected in 2003, we examine the poverty and vulnerability profiles of SHG and non-SHG rural, low-income household members. Our construction of the vulnerability is based on a methodology that allows for the estimation of household vul...

Skin cancer incidence has increased exponentially over the last three decades. In 2008 skin cancer caused 2280 deaths in the UK, with 2067 due to malignant melanoma. Early diagnosis can prevent mortality, however, conventional treatment requires multiple procedures and increasing treatment times. Second harmonic generation (SHG) imaging could offer diagnosis and demarcation of melanoma borders ...