We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa s...

AIM This study aims to develop and to standardize a polymerase chain reaction (PCR) assay that will diagnose clinical as well as carrier state of the disease and to compare the results with conventional microscopy technique. MATERIALS AND METHODS A herd of crossbred cattle with the previous history of theileriosis in village Lahli, district Rohtak, Haryana, was selected for this study. A tota...

RoBić, M., B. ARtuković, A. Beck, A. GudAn, A. SvetinA, Ž. GRABARević: Histopathological changes in stomachs of dogs with naturally acquired Helicobacter infection. vet. arhiv 77, 103-111, 2007. ABStrAct The aim of this study was to determine the presence of gastric Helicobacter-like organisms (GHLO) and consequent histopathological changes in dogs, and to evaluate different histological staini...

The value of different staining methods for rapid analysis of transbronchial needle aspirates during bronchoscopy has not been explored. In the present study, we compared a Papanicolaou-based rapid stain, prepared by a technologist and read by a cytopathologist, and a Wright-Giemsa-based rapid stain, prepared and read by a cytopathologist alone. Gold standard was the final laboratory report iss...

Parasites may lead bird species to extinction, affect host temporal and spatial population dynamics, alter community structure and alter individual' social status. We evaluated blood parasite prevalence and intensity according to bird families and species, among 925 birds that were caught in 2000 and 2001, in the Atlantic Forest in the State of Minas Gerais, Brazil. We applied Giemsa staining t...

The Papanicolaou stain is a gold-standard staining method for tumor diagnosis in human cytology. However, it has not been used routinely in veterinary cytology, because of its complicated multistep procedure and requirement for wet fixation. Currently, a rapid Papanicolaou stain using air-dried smears is utilized in human cytology, but usefulness of this rapid-air-dry Papanicolaou (RAD-Pap) sta...

background: trichomonas tenax, a commensal flagellated protozoan, inhabits in human oral cavity. this parasite is cos-mopoli­tan and frequently found in patients with poor oral hygiene and advanced periodontal disease. there is only one pub­lished study that rebound the prevalence of this parasite in iran. this pcr based study compared the prevalence of oral tricho­moni­asis in patients with or...

By determining the species-specific structure by morphological examination of sperm, it is possible to improve some disadvantages related short and long-term storage use in artificial insemination applications. When morphology canary spermatozoon evaluated, seen that has a long indented acrosome as well rather flagellum. To determine semen, methods used different poultry can be applied visually...

The aim of this research was to investigate the number prevalence Trypanosomiasis wild rats in Banyuwangi District. Sixty were trapped from 4 sub-districts, Banyuwangi, Srono, Songgon, and Tegaldlimo human residence, markets rice fields March until June 2017. Blood taken after anaesthetized using ether. examination parasite used blood smear Giemsa staining. result show that just 1 60 sample app...

Excess risk of leukemia and brain tumors after CT scans in children has been reported. We performed dicentric chromosome assay (DCAs) before and after CT scan to assess effects of low-dose ionizing radiation on chromosomes. Peripheral blood (PB) lymphocytes were collected from 10 patients before and after a CT scan. DCA was performed by analyzing either 1,000 or 2,000 metaphases using both Giem...