This paper describes the overnight Giemsa staining method for detecting mycoplasma infection of cell cultures. This simple method requires no special equipment and has practical application in most cell culture laboratories. This technique has been used conveniently to monitor stock cell cultures for mycoplasma infection over a 12-month period avoiding any contamination.

A direct Giemsa technique is described for the selective staining of Brd-U-substituted chromatin. It can advantageously be used for sister-chromatid exchange analysis and for the detailed cytological study of the mouse kinetochores. Presumptive inversions within the centromeric region of chromosomes 4 and 18 in the C57Black/6J mouse are described.

The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.

An exponential population of Saccharomyces cerevisiae cells was fractionated by centrifugal elutriation, using water as the elutriating liquid. Evidence that the population had been fractionated according to age in the cell cycle was obtained by examining the fractions for their size distribution, their microscopic appearance after Giemsa staining, and their ability to initiate synchronous growth.

The chromosomes of male and female individuals Enteromius callipterus [1] were assessed in this study with the objective determining diploid chromosome karyotype. samples obtained from Tahoss River, Jos Plateau, assessment was conducted using conventional Giemsa staining technique. reveals that individual E. has a number 2n = 50, fundamental autosomal arms (NFa) 82, karyotype formula 14M + 4m 3...

A total of 60 HIV infected patients complaining of dry cough for at least two weeks and attending the Out-patient Department of the Specialist Hospital, Waibargi, were screened for Pneumocystis carinii. Induced sputum samples were examined with Giemsa and Gomori silver methenamine stains. P. carinii were detected in 18 patients (30%) with silver stain and 13 patients (21.7%) with Giemsa stain. ...

Figure 1. May-Grünwald-Giemsa staining (original magnification, ϫ1000) of the patient's bone marrow aspirate (left panel), showing unstained oblong shapes (white arrows) in the cytoplasma of several macrophages, and Ziehl-Neelsen staining of the same area (right panel), revealing the unstained objects to be acid-fast bacilli (black arrows). Diagnosis: hemophagocytic syndrome due to disseminated...

AIM To assess five main histological features of gastritis in gastric mucosa colonized with Helicobacter pylori before and after the treatment. METHODS Histologic assessment of H. pylori-associated gastritis was performed according to the Sydney classification before and after the treatment in 97 patients. Two additional parameters - the presence of lymphocytic aggregates and coccoid forms of...

Micronuclei in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. To elucidate the effect of different staining procedures on the outcome of such investigation, we conducted a study in which the micronuclei frequencies in oral mucosa cells of heavy smokers (n = 20) and nonsmokers (n = 10) were evaluated with nonspecific (Giemsa, May-Grünwald-Giemsa) and DNA-spec...