A 2 × 2 factorial experiment was conducted to investigate the effects of cereal type (barley v. oat) and exogenous enzyme supplementation (with or without) on intestinal fermentation, and on indole and skatole levels in the intestinal content and the adipose tissue in finisher boars. The experimental treatments were as follows: (i) barley-based diet, (ii) barley-based diet with enzyme supplemen...

Full- and partial-length cDNAs encoding calmodulin mRNA have been cloned and sequenced from barley (Hordeum vulgare L.). Barley leaf mRNA, size-fractionated in sucrose density gradients, was used to synthesize double-stranded cDNA. The cDNA was cloned in lambdagt10 and screened with a synthetic, 14-nucleotide oligonucleotide probe, which was designed using the predicted coding sequences of the ...

Amarowicz R., Żegarska Z., Pegg R. B., Karamać M., Kosińska A. (2007): Antioxidant and radical scavenging activities of a barley crude extract and its fraction. Czech J. Food Sci., 25: 73–80. Phenolic compounds were extracted from the Candle variety of hull-less waxy barley with 80% (v/v) methanol to yield a crude preparation. Seven fractions (I–VII) were separated from the barley extract so ob...

Plants simultaneously interact with a plethora of species both belowground and aboveground, which can result in indirect effects mediated by plants. Studies incorporating plant genetic variation indicate that indirect effects mediated by plants may be a significant factor influencing the ecology and evolution of species within a community. Here, we present findings of a Quantitative Trait Locus...

MORC1 and MORC2, two of the seven members of the Arabidopsis (Arabidopsis thaliana) Compromised Recognition of Turnip CrinkleVirus1 subfamily ofmicrorchidiaGyrase,Heat ShockProtein90,HistidineKinase,MutL (GHKL)ATPases,were previously shown to be required in multiple layers of plant immunity. Here, we show that the barley (Hordeum vulgare) MORCs also are involved in disease resistance. Genome-wi...

Three hundred and sixty one-day-old chicks (Arian) were used in a completely randomized design with five treatments and four replicates for each treatment. The experimental treatments included: control group (without barley) with and without enzyme, 20% hulled barley with and without enzyme, 20% hull-less barley with and without enzyme. Body weights, feed intake and feed conversion were determi...

The aim of this study was to analyze the effect of different amounts of barley bran (0.3%, 0.6%, 0.9%, and 1.2%) on the viability of Lactobacillus acidophilus and the physicochemical and sensory properties of low-fat yogurt during storage period (28 days). Results showed that L. acidophilus number and viscosity in samples containing barley bran was significantly higher than the control group (p...

Full-length cDNA (FLcDNA) libraries consisting of 172,000 clones were constructed from a two-row malting barley cultivar (Hordeum vulgare 'Haruna Nijo') under normal and stressed conditions. After sequencing the clones from both ends and clustering the sequences, a total of 24,783 complete sequences were produced. By removing duplicates between these and publicly available sequences, 22,651 rep...

A collection of 5006 full-length (FL) cDNA sequences was developed in barley. Fifteen mRNA samples from various organs and treatments were pooled to develop a cDNA library using the CAP trapper method. More than 60% of the clones were confirmed to have complete coding sequences, based on comparison with rice amino acid and UniProt sequences. Blastn homologies (E<1E-5) to rice genes and Arabidop...

Barley quality and safety is affected by Fusarium both in the field and during post-harvest processes. Fusarium strains can survive, grow and produce mycotoxins during malting. We evaluated percentage of Fusarium infection (FI), and deoxynivalenol (DON) concentration in three, raw barley samples (high quality, naturallyinfected, F. graminearum inoculated barley) during various stages of malting...