BACKGROUND The purpose of this study was to determine the most appropriate dialysis equilibrium method to assess liposomal gel formulations containing hydrophobic drugs, to give the most accurate indication of drug release. METHODS Loperamide hydrochloride-encapsulated liposomes, composed of L-α-phosphatidylcholine and cholesterol (molar ratio of 2:1), were prepared according to the method of...

We present a stochastic model for single cell gel electrophoresis (COMET-assay) data. The distribution of length of DNA fragments is calculated according to a ’Random Breakage Model’ and the migration of DNA fragments among gel fibers is discussed. Essential to our approach is the use of point process structures, renewal theory and reduction to intensity histograms for further data analysis. Pa...

A medium containing 4% bio-trypcase and 1% yeast extract was used for the production of Staphylococcus aureus enterotoxin B. The yield obtained was estimated at 200 micrograms of enterotoxin per ml of S. aureus S-6 culture supernatant. The purification method involves chromatography on Biorex 70 resin, isoelectric focusing, and gel filtration on Sephadex G-100. The purified enterotoxin (isoioni...

BACKGROUND Why are metal nanoparticles sol-gel entrapped in ORMOSIL so active and stable? In other words, why ORMOSIL-entrapped metal nanoparticles are more active and selective than many heterogenized counterparts, including silica-entrapped noble metals? RESULTS Unveiling specific interactions between MNPs and the molecular structure of ORMOSIL, this work investigates subtle structural aspe...

Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting poin...

Alumina gels were prepared by the sol–gel method using alumatrane or tris(alumatranyloxy-i-propyl)amine as precursor synthesised directly from the reaction of inexpensive and readily available compounds, aluminium hydroxide and triisopropanolamine (TIS), via the one step process. Sol–gel process parameters, such as gel time, were correlated to variables of the initial stage of the process, such...

This work is a part of a study on the processing of fruit nectars enriched with Aloe vera gel. The aim of this study was to produce therapeutic and high nutritional mango nectar by supplementation of mango pulp with Aloe vera gel. Effects of different addition levels of Aloe vera gel (5, 10, 15, 20 and 25%) on the physical, microbial and chemical properties such as total soluble solids, total a...