A simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to Entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. The enzyme-linked immunosorbent assay is rapid (1 h 15 min, total incubation time), and th...

The dynamics of the sol/gel phase transition in agarose was analyzed with magnetic resonance elastography (MRE) and diffusion-weighted imaging, providing complementary information on a microstructural as well as on a macroscopic spatial scale. In thermal equilibrium, the diffusion coefficient of agarose is linearly correlated with temperature, independent of the sol/gel phase transition. In lar...

The aim of present study was to enhance topical permeation of clotrimazole gel preparation by using various permeability enhancers such as coconut oil, pistachio oil and sodium lauryl sulphate (SLS). Clotrimazole gel preparations were prepared and optimized by using three factor, five level central composite design. A second-order polynomial equation was generated in order to estimate the effec...

Studies of dynamic and equilibrium swelling of ionic gels are important in understanding the diffusion of physiologically important fluids in materials for site-specific controlled drug delivery applications. The dynamic and equilibrium swelling properties of dry glassy poly(2-hydroxyethyl methacrylate-co-methacrylic acid) and poly(2-hydroxyethyl methacrylate-co-acrylic acid) polymeric networks...

We investigate the behavior of the shear viscosity eta(p) and the mass-dependent diffusion coefficient D(m,p) in the context of a simple model that, as the cross link density p is increased, undergoes a continuous transition from a fluid to a gel. The shear viscosity diverges at the gel point according to eta(p) approximately (p(c)-p)(-s) with s approximately 0.65. The diffusion constant shows ...

The agar-gel precipitin-inhibition technique has been modified to detect antibodies of Histoplasma capsulatum in sera from human clinical cases and experimental animals infected with this organism. By use of this modified technique, the histoplasmosis can be detected more consistently and reliably than with the direct agar-gel diffusion test, and titers are comparable to those attained by the c...

We use simultaneous observation of translational and rotational Brownian motion of domains in lipid membranes to test the hydrodynamics-based theory for the viscous drag on the membrane inclusion. We find that translational and rotational diffusion coefficients of micrometer-sized solid (gel-phase) domains in giant unilamellar vesicles showing fluid–gel phase coexistence are in excellent agreem...