Helios Gene Gun-mediated transfection is a biolistic method for mechanical delivery of exogenous DNA into cells in vitro or in vivo. The technique is based on bombardment of a targeted cellular surface by micron- or submicron-sized DNA-coated gold particles that are accelerated by a pressure pulse of compressed helium gas. The main advantage of Helios Gene Gun-mediated transfections is that it ...

Male white-crowned sparrows have song "dialects," acquired in about the first 100 days of life by learning from older males. In the laboratory an alien white-crowned sparrow dialect can be taught. Once the song is established further acoustical experience does not change the pattern. White-crowned sparrows do not copy recorded songs of other sparrow species presented under similar conditions.

BACKGROUND To ascertain the prevalence of gun ownership, gun safety education, and parental attitudes on gun counseling in a Midwestern sample. METHODS Parents seeking care at participating practices in the Southwestern Ohio Ambulatory Research Network were recruited to complete a survey about gun ownership, gun safety education, and gun counseling attitudes. Attitudes and beliefs were compar...

Instrumentalists rely on physical gestures such as plunking, bowing, hitting, and blowing air into their instruments in order to produce sound; among tremendous number of human gestures, the body movements employed by musical performers are limited. This compositional project began with the idea of extending the relationship between human gestures and sound by electronic means. From that germin...

We define a new category of candidate tumor drivers in cancer genome evolution: 'selected expression regulators' (SERs)-genes driving dysregulated transcriptional programs in cancer evolution. The SERs are identified from genome-wide tumor expression data with a novel method, namely SPARROW ( SPAR: se selected exp R: essi O: n regulators identified W: ith penalized regression). SPARROW uncovers...

Abstract Recoil reduction is one of the most important issues for design aircraft gun. Traditional approaches this purpose will produce back-blast, which constitutes a hazard to fighter planes or attack helicopters. This paper presents closed type gas reflection device gun aiming reduce recoil force without ejecting propellant rearward. The launching process modeled by coupling classical interi...

BACKGROUND National surveillance systems have differentiated long guns into rifles and shotguns but fail to do so for handgun type. We sought to determine whether specific gun type data could be collected and whether knowledge of specific gun types (rifle, shotgun, pistol, revolver) could be used to distinguish gun homicide victims with respect to important injury parameters such as number of w...

T horrific loss of life at Sandy Hook Elementary School in Newtown, Connecticut, in December 2012 has prompted a national conversation about guns and mental illness in the United States. This tragedy occurred less than 6 months after 70 people were shot in a movie theater in Colorado and after highly publicized mass shootings in Arizona and at Virginia Tech. These four events share two common c...