Real-time quantitative PCR (RT-qPCR) has become a widely used method for exploring plant gene expression level. The requires using some stably expressed genes as reference to accurately normalize the RT-qPCR data. However, under various stresses and hormone treatments, levels of most vary. Environmental variations also influence their levels. lack validated, can mislead study function in pears....

Gene expression studies employing real-time PCR has become an intrinsic part of biomedical research. Appropriate normalization of target gene transcript(s) based on stably expressed housekeeping genes is crucial in individual experimental conditions to obtain accurate results. In multiple sclerosis (MS), several gene expression studies have been undertaken, however, the suitability of housekeep...

conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...

Coccidiosis is an intestinal disorder of poultry and often caused by simultaneous infections of several Eimeria species. GAPDH is one of the immunogenic common antigens among Eimeria tenella, E. acervulina, and E. maxima identified in our previous study. The present study was performed to further evaluate its immunogenicity and protective efficacy. The genes of GAPDH cloned from E. acervulina a...

conclusions according to the results of this study, quantification of hcmv and ebv observed in this study is high in periodontal tissue samples of severe chronic periodontitis. background although studies focused mainly on the identification of periopathogenic bacteria, recent reports have suggested that various herpes viruses may also be involved in the occurrence and progression of different ...

background and objectives:hbv and htlv-i are life threatening infectious agents in patients who receive blood and blood products. although serological methods have been proved to be useful, detection of these viruses has remained a challengingissue due to the many obstacles. by the advent of nucleic acid testing methods, especially in multiplex format, more precise detection is possible.the obj...

during 2009-10, real time rt-pcr and conventional rt-pcr techniques used for detecting btvs rna in 310 blood samples. for real time and gel based rt-pcr segment-1 and segment-10 selected as conserve genes to search any btv strains. using these methods, 58 (%18.7) and 14 (%4.5) positive samples were detected among the clinically suspected sheep. sensitivity of both molecular techniques evalua...

background: with the increasing concern for health and nutrition, dietary fat has attracted considerable attention. the composition of fatty acids in the diet is important because they are associated with major diseases including cancers, diabetes, and cardiovascular disease. the fatty acid synthase (fasn) and stearoyl-coa desaturase (delta-9-desaturase) (scd) genes affect fatty acid compositio...