introduction: infertility is a common phenomenon in modern societies. today, use of assisted reproductive technologies (arts) for treatment of infertility is common, and cryopreservation is one of these technologies. cryopreservation has impact on the function and percentage of fertility of human sperm. in this study, motility, viability, nitric oxide, and dna apoptosis were assessed before and...

The purpose of this study was to investigate the efficiency of the water test to evaluate the functional membrane integrity of ejaculated canine sperm after collecting, cooling, and freezing-thawing. Twenty-five ejaculates were obtained from 12 stud dogs by digital manipulation. Sperm-rich fractions were evaluated, diluted, cooled at 5oC and frozen-thawed. A conventional hypo-osmotic swelling t...

The aim of this study was to use biochemical markers to evaluate the quality of fresh and cryopreserved semen from the arctic fox (Vulpes lagopus). Twenty-three manually collected ejaculates were analysed for the main indicators of semen quality (sperm concentration and ejaculate volume). Sperm motility and percentage of morphologically normal and abnormal spermatozoa were determined according ...

The aim of this study was to define a simple extender for freezing rabbit semen from selected males used to inseminate selected doses to obtain embryos for an embryo bank. Four experiments were carried out. In the first experiment, freezing extender was defined on the basis of the results of the post-thawing motility rate. Three factors and their interactions were studied: final concentration o...

Differences in conception rate to first service between artificial inseminations (AI) carried out by commercial AI operators (CAI) or do-it-yourself operators (DIY), between natural service (NAT) and AI, between different AI sires, and between fresh and frozen-thawed semen, on Irish commercial dairy farms, were studied using logistic regression. The study comprised 12,933 potential first insemi...

Cryopreservation has the capacity to extend spermatozoa’s lifespan and viability. In addition, the semen samples can be collected, preserved and stored or sent to distant locations and still be used long after the death of the semen donor. In this study for the vitrification of dog sperm (fresh and swum-up sperm), different cryopreservation mediums on the basis of glycerol, milk and egg yolk we...