Rifampicin-loaded poly(ε-caprolactone)-b-poly(ethylene glycol)-poly(ε-caprolactone) flower-like polymeric micelles display low aqueous physical stability over time and undergo substantial secondary aggregation. To improve their physical stability, the lyoprotection-lyophilization process was thoroughly characterized. The preliminary cryoprotectant performance of mono- and disaccharides (e.g. ma...

The dermis in the holothurian body wall is a typical catch connective tissue or mutable collagenous tissue that shows rapid changes in stiffness. Some chemical factors that change the stiffness of the tissue were found in previous studies, but the molecular mechanisms of the changes are not yet fully understood. Detection of factors that change the stiffness by working directly on the extracell...

BACKGROUND We recently showed that freeze-dried cells stored for 3 years at room temperature can direct embryonic development following cloning. However, viability, as evaluated by membrane integrity of the cells after freeze-drying, was very low; and it was mainly the DNA integrity that was preserved. In the present study, we improved the cells' viability and functionality after freeze-drying....

The chloride diffusion of marine high-performance concrete under a couple actions, flexural load and freezing–thawing, was investigated by fast freezing thawing test in NaCl solution. Concrete specimens 100 × 515 mm were tested bending 300 freeze–thaw cycles the stress levels 15%, 30%, 50% ultimate fracture modulus. change microstructure analyzed SEM MIP. results indicated that coefficient coup...

Changes in sperm structure and function occur during the processing of semen. The present study was designed to investigate the effect on buck sperm during different stages of semen preparation including dilution, cooling, equilibration and freeze-thawing. Semen ejaculates from three mature bucks (replicates = 5) were diluted with tris-citric acid egg yolk glycerol extender at 37 ºC, cooled to ...

The occurrence of homologous interference in the replication of infectious pancreatic necrosis virus was demonstrated after successive passages of partially purified virus at high input multiplicities in trout and Atlantic salmon cell cultures. Pretreatment of cell cultures with interfering virus inhibited the replication of homologous standard infectious virus but not unrelated viruses. The ab...

The influence of aluminosilicates on the structure and drug release profiles of polyvinyl alcohol (PVA) – halloysite (HNT) hydrogels containing acetylsalicylic acid (ASA) as a model drug was monitored. The hydrogels were synthesized using a three cycle freeze – thawing procedure and were characterized by FTIR, XRD and SEM. The swelling degree and cytotoxicity were also determined. All hydrogels...

This paper is based on an experimental research that examined the effects of concrete`s major parameters on durability of concrete pavements and curbs under freezing and thawing cycles. These parameters include concrete mix design parameters such as water-cement ratio, fine aggregate percentage and using air entraining admixture and simulating real freeze-thaw cycles that infrastructures underg...

The cold climate is a favorable parameter for the development of tension cracks and decrease of rock brittleness. Therefore, this paper attempts to investigate the Hamekasi porous limestone in order to predict the brittleness indices during freeze-thaw cycles. The freeze–thaw test was executed for one cycle including 16 h of freezing, and 8 h of thawing. The geo mechanical properties and brittl...

The ability of cells to survive freezing and thawing is expected to depend on the physiological conditions experienced prior to freezing. We examined factors affecting yeast cell survival during freeze-thaw stress, including those associated with growth phase, requirement for mitochondrial functions, and prior stress treatment(s), and the role played by relevant signal transduction pathways. Th...