Inability to preserve airway mucus in situ has limited our understanding of its structure and function. This light- and transmission electron-microscopic study of rat tracheal mucus used a nonaqueous fixative that retains mucus (epiphase) over a lucent layer (hypophase). The fixative is a 1% solution of osmium tetroxide dissolved in a perfluorocarbon. The mean thickness of rat tracheal epiphase...

Background: In routine histopathology, 10% neutral buffered formalin (NBF) is the choice fixative. However, a human carcinogen, so there necessity for safer alternative. To best of our knowledge, honey, not natural or artificial has been tested to fix histological samples. This study determined effectiveness honey and other types fixatives tissues. <ns...

Potassium pyroantimonate, when used as fixative (saturated or half-saturated, without addition of any conventional fixative) has been demonstrated to produce intracellular precipitates of the insoluble salts of calcium, magnesium, and sodium and to preserve the general cell morphology. In both animal and plant tissues, the electron-opaque antimonate precipitates were found deposited in the nucl...

The multiplication of monolayer cultures of transformed rodent cells and normal or transformed human cells was not inhibited by concentrations of 7,12-dimethyIbenz(a)anthracene (DMBA) 200-500 times greater than those that inhibited the multiplication of normal embryonic rodent cells. Fluorescence microscopy showed that DMBA localized to about the same degree within the cytoplasm of cells that w...

The effect of three primary fixation procedures, used in the preparation of routine cytological samples: air-drying, Delaunay, and Saccomanno fixation, with postfixation in modified Böhm-Sprenger fixative, on nuclear features as a function of hydrolysis time is reported. Three different cell types: lymphatic cells (tonsil), epithelial cells (buccal mucosa) and mesenchymal cells (uterine myometr...

This study was designed to compare tissue preservation efficiency of formaldehyde substitution (FS) on cryosections with acetone (AC) and formaldehyde (FA). Brain, kidney, heart and liver had obtained from adult Dutch rabbit and were fixed with either FA or FS and AC prior to cryosectioning. Hematoxylin and eosin staining showed that AC is a poor fixative in preserving the general tissue and ce...

In this paper we report a qualitative morphological analysis of Wallerian degeneration in a marsupial. Right optic nerves of opossums Didelphis marsupialis were crushed with a fine forceps and after 24, 48, 72, 96 and 168 hours the animals were anaesthetized and perfused with fixative. The optic nerves were immersed in fixative and processed for routine transmission electron microscopy. Among t...