This paper reports the demonstration of degenerating myelin by the Marchi method in material kept in formalin for periods ranging from 22 months to over eight years. It has generally been believed that prolonged immersion in formalin renders material useless for investigation by the Marchi method, because of the constant occurrence of extensive artefacts. Fourteen days has usually been consider...

The formalin-Tween sedimentation method was compared with the formalin-ether sedimentation for parasitic detection. Of a total 297 fecal specimens examined, 72.1% were positive. The formalin-tween technique was effective for ascertaining helminths, particularly Ascaris lumbricoides, Trichuris trichiura and hookworm eggs; however it has less capability for protozoa detection. This method is simp...

in addition to its effect on reproductive organs, estrogen exhibits complex effects on brain structure, function and behavior. in this regard, the influence of gonadal hormones especially estrogens on nociception has recently been accepted. soybean is known as an important phytoestrogen containing plant that is used as an alternative for estrogen replacement therapy. in this study, the influenc...

objective(s): there are many reports about the role of rostral ventromedial medulla (rvm) in modulating stress-induced analgesia (sia). in the previous study we demonstrated that temporal inactivation of rvm by lidocaine potentiated stress-induced analgesia. in this study, we investigated the effect of permanent lesion of the rvm on sia by using formalin test as a model of acute inflammatory pa...

The formalin model is widely used for evaluating the effects of analgesic compounds in laboratory animals. Injection of formalin into the hind paw induces a biphasic pain response; the first phase is thought to result from direct activation of primary afferent sensory neurons, whereas the second phase has been proposed to reflect the combined effects of afferent input and central sensitization ...

Introduction: Uncoupling protein 2 (UCP2) in the inner mitochondrial membrane changes the activity of KATP channels and the cell excitability, probably by decreasing the ATP production. Given the expression of UCP2 in primary afferent neurons, and the importance of these channels in morphine-induced analgesia, genipin, an UCP2 inhibitor, may affect these processes, when administrated intratheca...