Abiotrophia adiacens and Abiotrophia defectiva, previously referred to as nutritionally variant streptococci, Streptococcus adjacens and Streptococcus defectivus, respectively, are causes of infective endocarditis. We describe a method of identifying these two species and also of distinguishing them from 15 other major etiological pathogens of infective endocarditis by means of 16S rRNA gene PC...

Abstract. The purpose of the research Was to study genetic diversity bovine leukemia virus isolates obtained in Belgorod Region using restriction fragment length polymorphism method (RFLP). Scientific novelty. Bovine leukosis is one most common chronic infectious diseases cattle many countries world, which causes significant economic losses livestock industry. typing (BLV), its structure, evalu...

Background: Quinolone resistant Salmonella serotypes have been reported in recent years and become increasingly widespread worldwide. Objectives: We evaluated the molecular mechanism of quinolone resistance non-typhoidal strains isolated from clinical samples Tehran, Iran. Methods: The present study included isolates originated hospitalized individuals outpatients Serotyping nalidixic acid-resi...

Moraxella (Branhamella) catarrhalis, a causative agent of otitis media, sinusitis, and exacerbation of bronchitis, has acquired widespread ability to produce beta-lactamase and can be nosocomially transmitted. The typing methods used in epidemiological analyses of M. catarrhalis are not optimal for genetic analyses. Two methods, a multiple-locus Southern blot (SB) method and a single-locus PCR-...

BACKGROUND Efficient monitoring of endemic and resurgent visceral leishmaniasis (VL) requires discriminatory molecular tools that allow direct characterization of etiological agents (i.e., the Leishmania donovani complex) in host tissues. This characterization is possible through restriction fragment-length polymorphism (RFLP) analysis of polymerase chain reaction (PCR)-amplified sequences (PCR...

Campylobacter hyointestinalis is considered as an emerging zoonotic pathogen. We have recently identified two types of cytolethal distending toxin (cdt) gene in C. hyointestinalis and designated them as Chcdt-I and Chcdt-II. In this study, we developed a PCR-restriction fragment length polymorphism (RFLP) assay that can differentiate Chcdt-I from Chcdt-II. When the PCR-RFLP assay was applied to...

polymorphism of the b-lactoglobulin (b-lg) gene in 101 cows belonging to the holstein herd and a superior cow, producing more than 150 kg milk/day, together with four offsprings was investigated by the polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method. in the holstein herd, the alleles a and b of the b-lg gene had frequencies of 0.53 and 0.47, respectively. th...

the present study investigated the calpain gene diversity in iranian one-hump camels (120 camels) using pcr-rflp method. three genotypes aa, ag and gg were observed with the frequencies of 0.02, 0.19 and 0.78, respectively. the frequencies of a and g alleles were 0.12 and 0.88, respectively. the chi-squared test didn’t show significant deviations from hardy-weinberg equilibrium (p<0.05). associ...

Adult and larval identification of Diabrotica can be difficult. Some adult identifications require considerable taxonomic experience while larvae of many Diabrotica species are morphologically indistinguishable. This study was conducted to determine whether 12 pest and non-pest Diabrotica species could be separated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RF...