Organic fluorophores embedded in lipid bilayers can nowadays be described by a multiscale computational approach. Combining different length and time scales, full characterization of the probe localization optical properties led to novel insight into effect environments. Following an introduction on advancements, three relevant probes are reviewed that delineate how approach lead probes' (non) ...

Mono- and di-boranil-substituted helicenes were prepared by BF2-borylation of the corresponding anils, readily synthesized condensation 2-amino- 2,15-diamino-helicenes with 4-(diethylamino)salicylaldehyde. After enantiomeric resolution using HPLC, their chiroptical properties including circularly polarized fluorescence in solution PMMA films investigated rationalized help NMR, X-ray quantum-che...

The microscopist’s utopia is filled with small, bright, photostable fluorescent probes. The contemporary toolbox of fluorescent probes—organic dyes, fluorescent proteins, and quantum dots—provides a range of sizes and photophysical properties, but we still await the perfect fluorescent probe. In a recent Biophysical Journal, Kim et al. (1) at the University of Illinois at Urbana-Champaign descr...

The use of fluorescent probes is one of the most powerful techniques for gaining spatial and temporal knowledge of dynamic events within living cells. Localized increases in the signal from cytosolic fluorescent protein constructs, for example, are frequently used as evidence for translocation of proteins to specific sites within the cell. However, differences in optical and geometrical propert...

To increase the diversity of fluorescent base analogues with improved properties, we here present the straightforward click-chemistry-based synthesis of a novel fluorescent adenine-analogue triazole adenine (A(T)) and its photophysical characterization inside DNA. A(T) shows promising properties compared to the widely used adenine analogue 2-aminopurine. Quantum yields reach >20% and >5% in sin...