In order to establish the effect of denervation on the structure of F-actin, polarisation of fluorescence of tryptophan residues of F-actin was determined in intact and denervated ghost muscle fibres (freed from myosin, troponin and tropomyosin) under various conditions. P\\, the polarisation parameter obtained by excitation of the fluorescence of tryptophan residues of F-actin by light polaris...

Polymerase chain reaction (PCR) is a major DNA amplification technology from molecular biology. The quantitative analysis of PCR aims at determining the initial amount of the DNA molecules from the observation of typically several PCR amplifications curves. The mainstream observation scheme of the DNA amplification during PCR involves fluorescence intensity measurements. Under the classical ass...

An antiserum to alpha-tocoquinone was prepared by immunization of rabbits. Immunization was obtained by injection of a conjugate consisting of the hapten alpha-tocoquinone attached to methylated ovalbumin into the rabbit. The antiserum recognizes the 3,4-dimethyl-p-benzoquinone group of the molecule as well as part of the immediate vicinity to the side chain. This is concluded from the fact tha...

Basics We will use the following parameters: • n, the security parameter.

Working memory capacity is one of the most important learner's characteristics that can be used as an input parameter for personalizing learning in order to improve the learning process. Therefore, knowing learner’s working memory capacity and personalizing the learning contents according to learner’s working memory capacity levels are important research issues. This paper proposes an approach ...

We perform a retrieval simulation on (1) how the fluorescence emission F s affects the retrieval of surface and scattering parameters from space-based observations of the O2A-band and on (2) how the retrieval of such parameters can mask the fluorescence signal. Here, we consider as retrieval parameters surface pressure ps, Lambertian surface albedo r, the aerosol optical density τ (AOD in main ...

We experimentally investigate trap-loss spectra of the cesium 6 S 1/2 ( F = 4) → 71 P 3/2 Rydberg transition by combining atomic magneto-optical trap with narrow-linewidth, continuously tunable 318.6 nm ultraviolet laser. Specifically, atoms in are excited to state due laser single-step excitation, which leads reduction fluorescence. Based on spectroscopy technology, Autler–Townes (AT) splittin...

BACKGROUND AND OBJECTIVE The clinical usefulness of 5-ALA guided detection of tumor tissue has been demonstrated for a number of malignancies. However, current techniques of intraoperative detection of protoporphyrin IX fluorescence in situ do not offer subcellular resolution. Therefore, discrimination of non-specific 5-ALA induced fluorescence remains difficult. MATERIALS AND METHODS In this...

The incubation of erythrocyte ghosts with mixtures of vitamins A+D3, A+E, A+K1 produces decrease or increase of fluorescence anisotropy r of the DPH probe, depending on the vitamin/vitamin ratio. We found a correlation between the order parameter S and cell fusion phenomena observed by phase contrast microscopy.

We have employed time dependent fluorescence polarization to investigate a main chain semiflexible polyester type liquid crystal polymer using the fluorescent probe 1,6-diphenylhexatriene. We have obtained the second rank order parameter and the tumbling reorientational time of the probeas a functionof temperature. Wehave found the polymer disordering to occur in a temperature range very close ...