The wild silkworm Bombyx mandarina is widely believed to be an ancestor of the domesticated silkworm, Bombyx mori. Silkworms are often used as a model for studying the mechanism of species domestication. Here, we performed transcriptome sequencing of the wild silkworm using an Illumina HiSeq2000 platform. We produced 100,004,078 high-quality reads and assembled them into 50,773 contigs with an ...

This work validates an in vitro observation that silk peptides derived from the α-chymotrypsin digestion of fibroin heavy chain promote wound healing properties. A molecular level evidence of binding of these peptide fragments to growth factors, their receptors and the corresponding ligand-receptor complexes has been provided. The strengths of interactions and possible conformational changes ca...

2. If tryptic hydrolysis is carried out in a medium containing 6 % of lithium thiocyanate no precipitation occurs, and the hydrolysate may be acidified and dialysed to give a solution of peptides of high molecular weight. 3. Further hydrolysis of this fraction with chymotrypsin shows that it consists predominantly of long sequences of amino acid residues throughout which glycyl residues alterna...

The development of a method for the quantitative determination sericin content ratio (SCR) is urgently needed silk refining and purification fibroin biomedical applications. In this work, series sericin/fibroin mixed samples with known SCRs were prepared by mixing initial extracted from Bombyx mori silk. Significant differences found in contents characteristic hydrophilic amino acids abundant h...

Silk fibroin, a natural multi-domain protein, has attracted great attention due to its superior mechanical properties such as ultra-high strength and stretchability, biocompatibility, as well as its versatile biodegradability and processability. It is mainly composed of β-sheet crystallites and amorphous domains. Although its strength is well known to be controlled by the dissociation of protei...

Currently silk fibroin is used more and in the biomedical researches, including a potential research direction creating wound dressing. Aloe vera gel has been as traditional herbal with many properties suitable for treatment of burns such anti-inflammatory, anti-bacterial, anti-fungal, especially improvable healing. Therefore, prepared fibroin/ film (FAV) was an ideal material dressings. In thi...

Electron microscope observations of thin sections of epoxy resin-embeded posterior silk gland cells at the later stage of the fifth instar revealed that the Golgi vacuoles and the secretory granules (fibroin globules) in the cytoplasm and the glandular lumen contain fine fibrous materials. In frozen thin sections these structures appear as electron-dense granules and electron-dense blocks, or a...

Abstract Natural silk fiber derived from the Bombyx mori (B. mori) silkworm has long been used as a luxury raw material in textile industry because of its shimmering appearance and durability, surgical suture for high strength flexibility. Regenerated fibroin, main protein extracted cocoons B. silkworm, recently gained considerable attention due to outstanding properties, including facile proce...

Plasma-assisted deposition is a facile, yet sophisticated method to form biocompatible coatings on materials and introduce specific surface interactions. The plasma process provides unique features such as activation, functionalization, assisted polymerization, all of which can be obtained under low power room temperature conditions. further provide with enhanced adhesion stability. Here, it re...