TCR gene rearrangement is strictly regulated during mouse ontogeny. The V-(D)-J junctions of alphabeta and gammadelta TCR transcripts expressed in the adult thymus are more highly diverse than those in the fetal thymus. We previously showed that adult hematopoietic stem cells (HSC) have a higher capacity to insert N nucleotides into Vgamma4 TCR transcripts than fetal HSC and that the level of N...

RATIONALE Fetal cells enter the maternal circulation during pregnancy and may persist in maternal tissue for decades as microchimeras. OBJECTIVE Based on clinical observations of peripartum cardiomyopathy patients and the high rate of recovery they experience from heart failure, our objective was to determine whether fetal cells can migrate to the maternal heart and differentiate to cardiac c...

A key question concerns the mechanisms that determine temporal identity in stem cells. Fetal hematopoietic stem cells (HSCs) differ from adult HSCs in terms of gene expression profile, surface marker expression, differentiation, and self-renewal capacity. We previously showed that the transcription factor SOX17 is expressed by fetal, but not adult, HSCs and is required for the maintenance of fe...

The contribution of distant and/or bone marrow-derived endogenous mesenchymal stem cells (MSC) to skin wounds is controversial. Bone marrow transplantation experiments employed to address this have been largely confounded by radiation-resistant host-derived MSC populations. Gestationally-acquired fetal MSC are known to engraft in maternal bone marrow in all pregnancies and persist for decades. ...

neurotrauma is one of the main medical problems that affect considerable number of population especially young persons in all countries. neurotrauma not only is a serious threat for life but also creates various disabilities and consequently imposes large costs to society. following central nervous system (cns) injury, tissue damage occurs via primary and secondary mechanisms. despite many atte...

Erythropoietic repopulating abilities of fetal liver cells and young and old adult marrow cells were compared as follows: Equal numbers of cells from a donor of each age were mixed with a constant portion of cells pooled from genetically distinguishable competitors. These mixtures were transplanted into stem cell-depleted recipients, and the proportions of recipient hemoglobin that were donor t...

in this study, our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented...

List of Abbreviations: EGP: Epidermal Growth Factor; FBS: Fetal Bovine Serum; FGF-2: Fibroblast Growth Factor-2; HGF-1: Hepatocyte Growth Factor-1; HSCs: Hematopoietic Stem Cells; IGF-1: Insulin-like Growth Factor; IL: Interleukin; MSCs: Mesenchymal Stem Cells; PBSCs: Peripheral Blood Stem Cells; PDGF: Platelet Derived Growth Factor; PRP: Platelet Rich Plasma; TGF-β: Transforming Growth Factor-...