Inhibitors of HMG-CoA reductase, namely statins, improve endothelial function independently of their cholesterol-lowering effects. Plasminogen activator inhibitor type-1 (PAI-1) plays a critical role in vascular pathophysiology both at the intraand extravascular levels. We therefore investigated the effects of atorvastatin (ATOR) and fluvastatin (FLU) on PAI-1 and also tissue-type plasminogen a...

Members of gram-negative bacteria family Pasteurellaceae, include a large number of important economically human and veterinary pathogens. Organisms belonging to the family can colonize in mucosal surfaces of the respiratory, alimentary, genital tracts and cause diseases in various mammals, birds, and reptiles. Hemorrhagic septicemia is an acute disease of cattle and buffaloes in tropi...

Cell CUltUreS. Pooled 11to 13-day mouse embryo minces in NCTC 135 medium supplemented with 10% fetal calf serum (Flow Laboratories) were used to initiate the cell cultures. The cultures were maintained in T-15 flasks and grown as mono layers. After the cell line was 90 days old, a substrain was established by supplementing the NCTC 135 medium with 10% horse serum; this resulted in conversion of...

background: free fetal dna (ffd) in maternal plasma/serum has increasingly become the source of fetal material for diagnostic purposes in recent years. this source of fetal material can be used for sex determination. rh typing paternally inherited sequences and compound heterozygosity. reports on the lack of consistent pcr amplification of y-chromosome sequences of ffd in maternal plasma/serum ...

CELL CULTURE: Human neuroblastoma SH-SY5Y cells were cultured in a mixture of Ham’s F12 medium and Eagle’s minimal essential medium (1:1 mixture), supplemented with 10% (v/v) fetal calf serum and 1% nonessential amino acids. Cells were incubated at 37 °C in a humidified incubator gassed with 95% air and 5% CO2, passaged every 7 days and used for up to 20 passages. Cells were harvested in phosph...

nowadays, cell -based and tissue engineered products have opened new horizons in treatment of incurable nervous system disorders. the number of studies on the role of schwann cells (sc) in treating nervous disorders is higher than other cell types. different protocols have been suggested for isolation and expansion of sc which most of them have used multiple growth factors, mitogens and fetal b...

The influence of aging on Schwann cell (SC) proliferation, migration and viability was studied in vitro. SCs were cultured in Ham F-10 medium enriched with 20% fetal calf serum (FCS), 40% FCS or collagen I gel plus 20% FCS. The migration of adult mice derived SCs was stimulated with FCS and collagen. With aging, SC migration, multiplication and viability decreased, indicating that ideal culturi...

19), and the high hydrolase levels may contribute to both decreased adhesiveness and abnormal migratory responses. These characteristics may, in addition, contribute to the malig nant behavior of tumor cells in vivo (16, 25). Following the work of Fidler (7) with the Bi 6 melanoma, several studies have shown that cultures of uncloned tumor cells contain phenotypically distinguishable subpopulat...