The aim of this study was to develop a novel microbially triggered and animal-sparing dissolution method for testing of nanorough polysaccharide-based micron granules for colonic drug delivery. In this method, probiotic cultures of bacteria present in the colonic region were prepared and added to the dissolution media and compared with the performance of conventional dissolution methodologies (...

Utilization of thin stillage (TS), derived from grain-based ethanol production, was investigated as an alternative source for microbial growth nutrients during direct conversion of cellulose by Clostridium thermocellum DSM 1237. Fermentation end-products synthesized by C. thermocellum grown on media prepared with various concentrations (50–400 g/L) of TS were compared to those synthesized by C....

Microbial media has undergone several changes since its inception but some key challenges remain. In recent years, there has been exploration of several alternative nutrient sources, both to cater to the specificity in requirement of growth of "fussy microorganisms" and also to reduce costs for large-scale fermentation that is required for biotechnology. Our mini-review explores these developme...

This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + ric...

The coagglutination (CoA) method for the identification of Neisseria gonorrhoeae colonies grown on selective culture media was used on 116 strains in a routine venereological laboratory together with the direct immunofluorescence (IF) test and the sugar fermentation reaction. Correlation of results between the CoA method and the direct IF test and between the CoA test and the sugar fermentation...

Gene expression profiles of a wine strain of Saccharomyces cerevisiae PYCC4072 were monitored during alcoholic fermentations with three different nitrogen supplies: (i) control fermentation (with enough nitrogen to complete sugar fermentation), (ii) nitrogen-limiting fermentation, and (iii) the addition of nitrogen to the nitrogen-limiting fermentation (refed fermentation). Approximately 70% of...

Over the last two decades, most of Italian vines have produced grapes with higher sugar to total acid ratios, greater concentrations of phenols and aromatic compounds and greater potential wine quality. As a consequence, the musts obtained by these grapes are more difficult to process because of the risk of slowing or stuck of fermentation. With the aim of describing the time evolution of the s...

[Objective] Screening and identification of a marine-derived actinomycetes with antifugal activity, and then to determine the optimum fermentation conditions. [Method] Candida albicans was used as indicator bacteria to screen active strains. Strain H74-21 was identified based on its morphological, cultural, physiological and sequence analysis of 16S rDNA. HPLC-MS was used to determine the optim...

Vinasse, a residue from bioethanol production containing high organic matter concentration, was used as substrate in submerged fermentation of Pseudomonas aeruginosa PA1 for biosurfactant production. About 2.7 g/L of rhamnolipids was obtained, with surface tension of 29.2 mN/m and critical micelle concentration of 80.3 mg/L. After separation of rhamnolipid and biomass, residual fermentation med...

The prehydrolyzate obtained from acid-catalyzed steam-exploded corn stover (ASC) mainly contains xylose and a number of inhibitory compounds that inhibit ethanol fermentation by Pichia stipitis. In this study, the effects of the ASC prehydrolyzate, specifically those of the carbohydrate-degradation products, lignin-degradation products (which were extracted from ASC prehydrolyzate using ethyl a...