Background: Interferon α-2b is a vital biotherapeutic produced through the recombinant DNA technology in E. coli. The recombinant IFN-α2b normally appears as intercellular IBs, which requires intensive refolding and purification steps. Method: Purification of IFN-α2b from solubilized IB was performed using two-phase extraction. To optimize refolding conditions, the effects of pH and different a...

Plant density was varied with P, Ca, Mg, and KNO3 in a multifactor experiment to improve Curcuma longa L. micropropagation, biomass and microrhizome development in fed-batch liquid culture. The experiment had two paired D-optimal designs, testing sucrose fed-batch and nutrient sucrose fed-batch techniques. When sucrose became depleted, volume was restored to 5% m/v sucrose in 200 ml of modified...

A mathematical model for fed-batch culture of hybridoma cells [24] has been employed for generating simulation data in this study. The model is a seventhorder nonlinear model where both glucose and glutamine concentrations are used to describe the specific growth rate, μ. The cell death rate, kd, is governed by lactate, ammonia and glutamine concentrations. The specific MAb production rate, qMA...

On-line monitoring of batch processes using multi-way independent component analysis has attracted much attention in both academia and industry. This paper focuses on two knotty issues concerning selecting dominant independent components without a standard criterion and determining the control limits of monitoring statistics in the presence of non-Gaussian distribution. To optimize the number o...

Background Visceral leishmaniasis is an infecto-parasitarian disease caused by obligatory intracellular protozoa belonging to the genus Leishmania, and might be lethal since there is no precocious diagnosis and proper treatment. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Genetically modified microorganisms are used industrially in general for prod...

Hybrid modeling, meaning the integration of data-driven and knowledge-based methods, is quickly gaining popularity in many research fields, including bioprocess engineering development. Recently, part hybrid methods have been largely extended with machine learning algorithms (e.g., artificial neural network, support vector regression), while mechanistic typically based on differential equations...

Xanthan gum is an extracellular hetero polysaccharides of many industrial applications and produced by Xanthomonas campestris. This biopolymer is produced as extracellular product in submerged fermentation system. The production of xanthan was influenced by the concentration in glucose in medium. The maximal volumetric production of 17.9 g L was obtained in 80 g L glucose culture in shake flask...

Safe and optimal operation of complex production processes is one of the most important research and development problems in process engineering. This problem is the most relevant at the design of the optimal feeding profile of fed-batch chemical reactors due to the nonlinear and unstable dynamical behavior of the processes. This paper shows that how the optimal feeding policy can be determined...

Perfusion culture optimization in multiple noninstrumented small-scale flasks allows reduced expense and time associated with process development. These cultures normally use a different process mode because at small scales it is not practical to retain the cells for medium perfusion. In this work, the kinetics of growth, nutrient consumption, metabolite, and product formation were compared in ...