Saccharamyces cerevisia known as baker’s yeast is a product used in various food industries. Worldwide economic competition makes it a necessity that industrial processes be operated in optimum conditions, thus maximisation of biomass in production of saccharamyces cerevisia in fedbatch reactors has gained importance. The facts that the dynamic fermentation model must be considered as a constra...

Considering the hybrid nature in fed-batch culture of glycerol biconversion to 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae, we propose a state-based switching dynamical system to describe the fermentation process. To maximize the concentration of 1,3-PD at the terminal time, an optimal switching control model subject to our proposed switching system and constraints of continuous state ine...

Improved fermentation processes were developed for the production of mannitol by a heterofermentative lactic acid bacterium (Lactobacillus intermedius NRRL B-3693). A fed-batch fermentation protocol overcame limitations caused by high substrate concentrations. The process was developed using corn steep liquor and glucose as inexpensive industrial nutrient sources, supplemented with a small amou...

Lignocellulose is one of the most abundant renewable feedstocks that has attracted considerable attention as a substrate for biofuel and biochemical production. One such biochemical product, lactic acid, is an important fermentation product because of its great potential for the production of biodegradable and biocompatible polylactic acid. High-titer lactic acid production from lignocellulosic...

This paper presents a unifying methodology for optimization of biotechnological processes, namely optimal adaptive control, which combines the advantages of both the optimal control and the adaptive control approaches. As an example, the design of a substrate feeding rate controller for a class of biotechnologlcal processes in stirred tank reactors characterized by a decoupling between biomass ...

This study focuses on improving the productivity of a fed-batch ethanol fermentation process by developing and implementing in real time, an optimum feeding policy. A constraint-based stoichiometric model is developed using the systems biology approach, which can quantitatively predict the cellular behavior of the E coli strain, KO11. However, the predictions from this model are accurate under ...

Simulation software based on a detailed unstructured model for penicillin production in a fed-batch fermentor has been developed. The model extends the mechanistic model of Bajpai and Reuss by adding input variables such as pH, temperature, aeration rate, agitation power, and feed flow rate of substrate and introducing the CO2 evolution term. The simulation package was then used for monitoring ...

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...

In this work, Evolutionary Algorithms (EAs) are used to control a recombinant bacterial fed-batch fermentation process, that aims to produce a bio-pharmaceutical product. Initially, a novel EA was used to optimize the process, prior to its run, by simultaneously adjusting the feeding trajectory, the duration of the fermentation and the initial conditions of the process. Finally, dynamic optimiz...