Nucleobase analogs 5-methylisocytosine ((Me)isoC) and isoguanine (isoG) form a non-natural base pair in duplex nucleic acids with base pairing specificity orthogonal to the natural nucleobase pairs. Sequencing reactions were conducted with oligodeoxyribonucleotides (ODNs) containing d(Me)isoC and disoG using modified pyrosequencing and dye terminator methods. Modified dye terminator sequencing ...

Hsp90 is a conserved chaperone that facilitates protein homeostasis. Our crystal structure of the mitochondrial Hsp90, TRAP1, revealed an extension of the N-terminal β-strand previously shown to cross between protomers in the closed state. In this study, we address the regulatory function of this extension or 'strap' and demonstrate its responsibility for an unusual temperature dependence in AT...

Mechanical stretching of self-interacting homopolymers is studied through molecular dynamics simulations in which the polymers are pulled with constant speed. At temperatures below the compactification temperature, the force-extension curves show a plateau that corresponds to the situation in which the polymer adopts "ball-string" configurations. The dependence of rupture distances on contact o...

Farmers' perception of the environmental impacts of modern agricultural technology diffusion and factors determining such awareness were examined using survey data from 21 villages in three agro-ecological regions of Bangladesh. Results reveal that farmers are well aware of the adverse environmental impacts of modern agricultural technology, although their awareness remains confined within visi...

In this chapter we introduce a model for analyzing the spread of epidemics in a disconnected mobile network. The work is based on an extension, to a dynamic setting, of the eigenvector centrality principle introduced by two of the authors for the case of static networks. The extension builds on a new definition of connectivity matrix for a highly partitioned mobile system, where the connectivit...

BACKGROUND Coinfection with human immunodeficiency virus (HIV) may modify the risk of transmitting tuberculosis. Some previous investigations suggest that patients coinfected with HIV and tuberculosis are less likely to transmit infection, whereas others do not support this conclusion. Here, we estimated the relative risk of tuberculosis transmission from coinfected patients compared to HIV-neg...

Background Previous studies of Greenlandic children's disease pattern and contacts to the health care system are sparse and have focused on the primary health care sector. Objective We aimed to identify the disease pattern and use of health care facilities of children aged 0-10 in two Greenlandic cohorts. Methods and design In a retrospective, descriptive follow-up of the Ivaaq (The Greenland C...

BACKGROUND The characterization of protein-peptide interactions is a challenge for computational molecular docking. Protein-peptide docking tools face at least two major difficulties: (1) efficient sampling of large-scale conformational changes induced by binding and (2) selection of the best models from a large set of predicted structures. In this paper, we merge an efficient sampling techniqu...

BACKGROUND Although children in contact with adults with tuberculosis (TB) should receive isoniazid (INH) preventive therapy (IPT), this is rarely implemented. OBJECTIVE To assess whether a community-based approach to provide IPT at the household level improves uptake and adherence in Ethiopia. METHODS Contacts of adults with smear-positive pulmonary TB (PTB+) were visited at home and exami...