Objective(s): Honey’s ability to kill microorganisms and even eradication of chronic infections with drug-resistant pathogens has been documented by numerous studies. The present study is focused on the action of honey in its sub-inhibitory levels to impact on the pathogens coordinated behaviors rather than killing them. Materials and Methods:</strong...

When environmental conditions deteriorate and become inhospitable, generic survival strategies for populations of bacteria may be to enter a dormant state that slows down metabolism, to develop a general tolerance to hostile parameters that characterize the habitat, and to impose a regime to eliminate damaged members. Here, we provide evidence that the pseudomonas quinolone signal (PQS) mediate...

Within recent years, it has been established that extracellular DNA is a key constituent of the matrix of microbial biofilms. In addition, it has recently been demonstrated that DNA binds positively charged antimicrobials such as aminoglycosides and antimicrobial peptides. In the present study, we provide evidence that extracellular DNA shields against aminoglycosides in Pseudomonas aeruginosa ...

Pseudomonas aeruginosa by an OprD-mediated mechanism. 2 3 4 Jerónimo Rodríguez-Beltrán1,2, Gabriel Cabot3, Estela Ynés Valencia1,2,&, Coloma 5 Costas1,2, German Bou4, Antonio Oliver3 and Jesús Blázquez1,2 *. 6 7 8 1: Instituto de Biomedicina de Sevilla (IBIS), Consejo Superior de Investigaciones 9 Científicas (CSIC) and Universidad de Sevilla, Sevilla, Spain. 10 2: Centro Nacional de Biotecnolo...

An in vitro cytotoxicity microassay for the measurement of nanogram quantities of Pseudomonas aeruginosa exotoxin and Vibrio cholerae enterotoxin is described.

A simple hemagglutination inhibition (HI) test for the serological diagnosis of toxoplasmosis has been developed and evaluated. A total of 84 human and 120 mouse serum samples were tested by the newly developed HI test and compared with an immunoglobulin G-indirect fluorescent antibody test. Statistical analysis of serum titers obtained by using the HI test and the immunoglobulin G-indirect flu...