نتایج جستجو برای: exosome

تعداد نتایج: 3188  

Journal: :The EMBO journal 1999
C Allmang J Kufel G Chanfreau P Mitchell E Petfalski D Tollervey

The yeast nuclear exosome contains multiple 3'-->5' exoribonucleases, raising the question of why so many activities are present in the complex. All components are required during the 3' processing of the 5.8S rRNA, together with the putative RNA helicase Dob1p/Mtr4p. During this processing three distinct steps can be resolved, and hand-over between different exonucleases appears to occur at le...

2017
Birgit Märtens Linlin Hou Fabian Amman Michael T. Wolfinger Elena Evguenieva-Hackenberg Udo Bläsi

The conserved Sm and Sm-like proteins are involved in different aspects of RNA metabolism. Here, we explored the interactome of SmAP1 and SmAP2 of the crenarchaeon Sulfolobus solfataricus (Sso) to shed light on their physiological function(s). Both, SmAP1 and SmAP2 co-purified with several proteins involved in RNA-processing/modification, translation and protein turnover as well as with compone...

2012
Olivier G. de Jong Marianne C. Verhaar Yong Chen Pieter Vader Hendrik Gremmels George Posthuma Raymond M. Schiffelers Marjan Gucek Bas W.M. van Balkom

BACKGROUND The healthy vascular endothelium, which forms the barrier between blood and the surrounding tissues, is known to efficiently respond to stress signals like hypoxia and inflammation by adaptation of cellular physiology and the secretion of (soluble) growth factors and cytokines. Exosomes are potent mediators of intercellular communication. Their content consists of RNA and proteins fr...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2005
Alexei V Kazantsev Angelika A Krivenko Daniel J Harrington Stephen R Holbrook Paul D Adams Norman R Pace

The x-ray crystal structure of a 417-nt ribonuclease P RNA from Bacillus stearothermophilus was solved to 3.3-A resolution. This RNA enzyme is constructed from a number of coaxially stacked helical domains joined together by local and long-range interactions. These helical domains are arranged to form a remarkably flat surface, which is implicated by a wealth of biochemical data in the binding ...

Journal: :Science-Business eXchange 2008

Journal: :Cell 1999

Journal: :RNA 2017
Sebastian Falk Jan-Niklas Tants Jerôme Basquin Matthias Thoms Ed Hurt Michael Sattler Elena Conti

The nuclear exosome and the associated RNA helicase Mtr4 participate in the processing of several ribonucleoprotein particles (RNP), including the maturation of the large ribosomal subunit (60S). S. cerevisiae Mtr4 interacts directly with Nop53, a ribosomal biogenesis factor present in late pre-60S particles containing precursors of the 5.8S rRNA. The Mtr4-Nop53 interaction plays a pivotal role...

Journal: :The Biochemical journal 2004
Karine Laulagnier Claude Motta Safouane Hamdi Sébastien Roy Florence Fauvelle Jean-François Pageaux Toshihide Kobayashi Jean-Pierre Salles Bertrand Perret Christian Bonnerot Michel Record

Exosomes are small vesicles secreted from multivesicular bodies, which are able to stimulate the immune system leading to tumour cell eradication. We have analysed lipids of exosomes secreted either upon stimulation from rat mast cells (RBL-2H3 cells), or constitutively from human dendritic cells. As compared with parent cells, exosomes displayed an enrichment in sphingomyelin, but not in chole...

Journal: :The Journal of biological chemistry 2001
R Brouwer C Allmang R Raijmakers Y van Aarssen W V Egberts E Petfalski W J van Venrooij D Tollervey G J Pruijn

The yeast exosome is a complex of 3' --> 5' exoribonucleases. Sequence analysis identified putative human homologues for exosome components, although several were found only as expressed sequence tags. Here we report the cloning of full-length cDNAs, which encode putative human homologues of the Rrp40p, Rrp41p, and Rrp46p components of the exosome. Recombinant proteins were expressed and used t...

Journal: :RNA 2012
Shakir Sayani Guillaume F Chanfreau

The nuclear exosome and the nonsense-mediated mRNA decay (NMD) pathways have been implicated in the degradation of distinct unspliced transcripts in Saccharomyces cerevisiae. In this study we show that these two systems can act sequentially on specific unspliced pre-mRNAs to limit their accumulation. Using steady-state and decay analyses, we show that while specific unspliced transcripts rely m...

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