background: human leukocyte antigen-e (hla-e)is a non-classical major histocompatibility complex (mhc) class i antigens which expressed on extra villous cytotrophoblast, which interacts with nkg2a, is an inhibitory receptor on natural killer (nk) cells and leading to down regulation of immune response in the maternal-fetal interface and provides maternal immune tolerance of the fetus. objective...

background: cystic echinococcosis caused by the metacestode of echinococcus granulosus is a major problem in both humans and domestic animals health. there-fore, a standardized and approachable diagnostic tool (rapid tests) for the serodiag-nosis of cystic echinococcosis (ce) is still needed. methods: in the present work, antigen b labeled with gold nanoparticles was used to detect antibodies a...

dna coding for the core antigen from hepatitis b virus (hbcag) was amplified, cloned and propagated in e. coli. the core protein was expressed in e. coli and the product was readily detected by western blot. this protein can be used as a diagnostic material in serum screening tests. to increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

Background: Monoclonal antibodies (mAbs) are essential tools for many molecular im-munology investigations, epitope mapping and molecular modelling, clinical laboratory di-agnostic tests and immunotherapy. Humoral immune response of immunized animals largely depends on the nature of antigen and the immunization technique. Polysaccharides and heavily-glycosylated proteins are very elusive target...

Particulate antigen-delivery systems are instrumental for therapeutic vaccination, aiming at improving the safety and efficacy of treatments by targeting specialized antigen-presenting cells (APCs). However, induction potent adaptive immune responses, especially cellular immunity, remains a major challenge. Herein, novel nanoparticulate system based on amphiphilic cyclodextrins (CDs) is develop...

Abstract Multi-analyte single cell technologies have potential to greatly accelerate antibody discovery by identifying antigen-specific B cells. Barcode Enabled Antigen Mapping (BEAM) provides a high-throughput, multimodal analysis of antigen-bound cells (BEAM-Ab) using 10x Genomics Chromium Single Cell Immune Profiling v2 Solution and novel computational approaches discover B-cell receptor (BC...

Leptospirosis is one of the most prevalent zoonotic diseases worldwide. Currently, multivalent whole-cell leptospiral vaccines can induce protection against leptospirosis. Therefore, preparation and formulation of new generations of vaccines that can stimulate long-term immunity for leptospirosis control are essential. The aim of this study was to prepare and characterize alginate microspheres ...