We present whole genome profiling (WGP), a novel next-generation sequencing-based physical mapping technology for construction of bacterial artificial chromosome (BAC) contigs of complex genomes, using Arabidopsis thaliana as an example. WGP leverages short read sequences derived from restriction fragments of two-dimensionally pooled BAC clones to generate sequence tags. These sequence tags are...

relies on all proteins from an organism being in sequence databases. In this manner, if only one protein within a given pI and mass range is found with a certain aminoor carboxy-terminal sequence tag, one can be confident that there is no other, as yet undescribed, protein that could otherwise match the tag. In fully sequenced organisms, the procedure is thus self-checking. The specificity of s...