A common feeding practice during the dry period is to switch dairy cows to an energy-dense diet 3 wk prepartum, but this practice may lead to the overconsumption of energy and increase the risk of metabolic disease postpartum. The aim of this trial was to compare the metabolic status of transition Holstein dairy cows fed a 77% forage diet (77F; NEl = 1.46 Mcal/kg; NDF = 41%) vs. those fed an 87...

ANALYSIS OF NON-ESTERIFIED FATTY ACIDS IN HUMAN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY WITH SPLITLESS INJECTION. The aim of the present work was to test the combination of non-esterified fatty acid (NEFA) isolation using fumed silicon dioxide with capillary gas-chromatography (C-GC) with splitless injection for the analysis of NEFAs in human plasma. Injection volume, solvent re-condensation and...

I. Prolonged intermittent exposure to reduced environmental temperature (+ 2") produced in rats given a nutritionally well-balanced diet a decrease in the concentration of esterified fatty acids in the blood serum, liver and epididymal fat tissue. In the last there was a significant increase in the unesterified: esterified fatty acid ratio. The hepatic synthesis of fatty acids from [1-14C]aceta...

the purpose of this research was the study of reproductive performance in postpartum dairy cows with different parities. twenty holstein dairy cows were assigned into two equal primiparous and multiparous groups. body weight and body condition score (based on 1-5 scoring) were measured every other week, starting from two weeks before calving till pregnancy confirmation. daily milk yield was als...

Three major regulatory steps can be recognized in the control of ketogenesis (Fig. 1): (1) regulation of the supply of the major ketogenic substrate, plasma non-esterified fatty acids, by control of their release from adipose tissue; (2) regulation in the liver of the relative fluxes of non-esterified fatty acids through the pathways of /3-oxidation and esterification; (3) regulation in the liv...

In the suckling newborn rat, blood ketone bodies begin to increase slowly 4h after birth and then rise sharply between 12 and 16h, whereas the major increase in plasma non-esterified fatty acids and liver carnitine occurs during the first 2h of life, parallel with the onset of suckling. In the starved newborn rat, which shows no increase in liver carnitine unless it is fed with a carnitine solu...

...................... • ...................... . Acknowledgements .....••••.•.•...••.....•..•.•.•...•..•. 2 Introduction ........................................... 3 Materials and Methods .......•....•.....••.•...••.•.••.. 7 Results ....••......•.•.....•..•.........••••.. o••••••••14 Di scl1ss ion .•...•....•••••...•....•....••••.•.......•... 17 Summary • ••••.•.••••••••.••.•..•.••.....•.•.•....

The objective of the study was to evaluate the relationships between body condition and lymphocyte functions in periparturient dairy cows. Thirty days before expected calving, 21 Holstein cows were categorized as thin (n = 6), medium (n = 8), or overconditioned (n = 7) based on body condition score (BCS). Blood samples were collected on 21, 14, 7, and 3 d before calving and on d 3, 7, 14, 21, 2...