In recent years, valuable experience and insights have been gained into L-forms (cell-wall-deficient variants) of bacteria fungi their disease-trigger potential in cases with chronic infections, autism spectrum disorders, autoimmune neurodegenerative diseases. Based on the concept “internal” blood microbiota, consisting its relevance to health disease, current study aims outline profile dysbiot...

F18 fimbrial Esherichia coli strains are associated with porcine postweaning diarrhea and pig edema disease. Recently, the FedF subunit was identified as the adhesin of the F18 fimbriae. In this study, adhesion domains of FedF were further studied by constructing deletions within the fedF gene and expressing FedF proteins with deletions either together with the other F18 fimbrial subunits or as...

In the present investigation, a gene encoding extracellular lipase was cloned from a Bacillus licheniformis. The recombinant protein containing His-tag was expressed as inclusion bodies in Esherichia coli BL21DE3 cells, using pET-23a as expression vector. Expressed protein purified from the inclusion bodies demonstrated ~22 kDa protein band on 12 % SDS-PAGE. It exhibited specific activity of 0....

Live-cell imaging by light microscopy has demonstrated that all cells are spatially and temporally organized. Quantitative, computational image analysis is an important part of cellular imaging, providing both enriched information about individual cell properties and the ability to analyze large datasets. However, such studies are often limited by the small size and variable shape of objects of...

Gene-mediated enzyme prodrug therapy (GDEPT) seeks to increase the therapeutic index of anti-neoplastic agents by promoting selective activation of relatively nontoxic drug derivatives at sites of specific enzyme expression. Glucuronide prodrugs are attractive for GDEPT due to their low toxicity, bystander effect in the interstitial tumor space and the large range of possible glucuronide drug t...

Baobab (Adansonia digitata L.) is known in Africa as the tree of life because there are many traditional uses for every part of it, from the leaves to the roots. This study carried out the proximate analysis of baobab leaves, screened the phytochemicals present and determined the leaves’ aqueous extract antimicrobial activity to suit the traditional way of preparation and use. Phytochemicals sc...

The article presents a comparison between microbiological and high performance liquid chromatographic (HPLC) assays for quantification of moxifloxacin in tablets, ophthalmic solutions and human plasma. The microbiological method employed a cylinder-plate agar diffusion assay using a strain of Esherichia coli ATCC 25922 as the test organism and phosphate buffer (pH8) as the diluent. The calibrat...

The DNA-binding mode of archaeal feast/famine-regulatory proteins (FFRPs), i.e. paralogs of the Esherichia coli leucine-responsive regulatory protein (Lrp), was studied. Using the method of systematic evolution of ligands by exponential enrichment (SELEX), optimal DNA duplexes for interacting with TvFL3, FL10, FL11 and Ss-LrpB were identified as TACGA[AAT/ATT]TCGTA, GTTCGA[AAT/ATT]TCGAAC, CCGAA...

An experiment was conducted in the Biological Dep. College of Science, Kufa University to determine the antagonism activity of Lemon and Lime juices against some bacteria e.g. Esherichia coli, Klebsiella pneumonia, and Shigella flexneri. Results of this experiment revealed that all juices concentrations have inhibiting effects against bacteria due to the presence of citric acid and some volatil...