conclusions this study suggests that epec strains are important contributors to diarrhea in iranian children. because of the weakness of routine microbiological tests and poor specifity of serological tests, it is recommended that the epec strains are better detected by molecular methods. results the e. coli isolates were confirmed by the presence of the uida gene. the epec was diagnosed in 6 c...

diarrhea is an important hygienic problem in under development countries. among pathogenic agents escherichia coli is the most etiologic agent of diarrhea in children below five years old. some e. coli strains produce cytotoxins that have cytopathic effect on vero cell and identified as verotoxin. this study performs for resolve frequency of vero toxigenic escherichia coli (vtec) in kerman city...

conclusions overall, except for pcvd432 and aggr, we detected high heterogeneity of virulence factors among eaec isolates causing diarrhea in children. one set of genes, in the combination, pic- sepa- agg4a, was associated with strong biofilm. results among the 322 e. coli isolated, 23 were identified as eaec. all eaec carried a 630-bp fragment of the plasmid (paa) encoded pcvd432 and aggr gene...

introduction : toxoplasmosis is a parasitic infection caused by the protozoan toxoplasma gondii it leads to serious medical problems in congenitally-infected and immunocompromised individuals, while it is quite harmless in immunocompetent individuals. toxoplasma tissue cyst matrix protein (mag1) induces early humoral and cell-mediated immune responses. previous studies suggested recombinant mag...

introduction: escherichia coli is one of the important pathogens in human with global importance. because of the necessity for identification and the use of novel antibacterial compounds against e. coli , in this present study we focused on the antibacterial effects of synthesized thiazole, imidazole and tetrahydropyridine derivatives on e. coli .   methods : for evaluation of antibacterial eff...

background:  one of the most common infections in human is urinary tract infection  (uti)  and  uropathogenic   escherichia  coli   is  one  of  its  major causative agents . uti is extremely common among young women. children under age 5 are also highly at risk. considering the prevalence of this disease, it is necessary  to design  an appropriate  diagnostic  method  for its effective diagnos...

background: acute infectious diarrhea is one of the most important causes of morbidity and mortality worldwide. objectives: the objective of this study was to characterize antimicrobial resistant diarrheagenic escherichia coli strains isolated from diarrheic children in tehran, ir iran. patients and methods: in total, 550 stool samples from diarrheic pediatric patients, aged less than 60 months...

background and objective: enterohemorrhagic escherichia coli (ehec) o157:h7 is an important enteric pathogen in human causing bloody or nonbloody diarrhea, which may be complicated by hemolytic uremic syndrome (hus). cattle are an important reservoir of ehec. this research aims at vaccination with a divalent chimer protein composed of espa 120 and intimin 282 and its preventive effect of ehec o...

background and objectives: pathogenic strains of escherichia coli are a common cause of acute infectious diarrhea. the aim of this study was to investigate the frequency, virulence markers and antibiotic resistance patterns of diarrheagenic e. coli (dec) isolated from adolescents and adults in hamadan, west of iran. materials and methods: a total of 187 stool samples were collected from adults ...

reteplase is a part of tissue plasminogen activator (t-pa) used for theremoval of thrombi in blood vessels. in the present study we express the reteplase genein escherichia coli top10 and then its thrombolytic activity was measured. the recombinant plasmid pbadgiiia was transformed into the competent escherichia coli top10 and then transformed bacteria was seeded into bioreactor containing 1.5 ...