rosine B, Tartrazine, Lissamine Green B, Pyronin G, Acetyl Yellow G, Ponceau 6R, Ponceau SX, Food Orange #2, and Food Yellow #3. The concentration of dye tested was 50 mg/ L of incubation mixture (25 pg/tube) in RIA systems for human lutropin (hLH), its alpha and beta subunits, rat LII, rat corticotropin (rACTH), rat insulin, and human thyroxin (T4) and testosterone. (For T4 we usedthe MAGIC-T4...

In this "phosphoroimmunoassay," a phosphorescent label is used: erythrosin (tetraiodofluorescein). Its long-lived phosphorescence was detected after pulsed excitation in a time-resolved luminescence spectrometer. To achieve convenient open-atmosphere phosphorimetry in liquid solution at room temperature, we used sodium sulfite as an "in situ" chemical deoxygenator, to eliminate oxygen quenching...

A method is proposed for measuring the fluorescence quantum yield (QY) using a commercial spectrophotometer with a 150 mm integrating sphere (IS) detector. The IS detector is equipped with an internal cuvette holder so that absorbance measurements can be performed with the cuvette inside the IS. In addition, the spectrophotometer has a cuvette holder outside the IS for performing conventional a...

The susceptibility of thymocytes from STS/A to radiation-induced cell death was compared with that of thymocytes from BALB/cHeA. After in vitro exposure to 12 Gy X-ray, thymocytes were incubated at 37 degrees C for 8 h and then cell mortality was assessed by 0.02% erythrosin B exclusion. Cell death took place from 2 h in the incubation period, reaching a maximum at 6 h for both strains. The dos...

Solutions of these resins were also used in making permanent mounts of moulds. The slide was first covered with a uniform layer of the resin in precisely the same manner as described above. The film was then air dried for two to three minutes, at the end of which time it still presented a slightly sticky surface. This surface was then impregnated with the fungus in one of two ways. Either the r...

The chemical action of photodynamic substances is very wide. Not only do they cause alteration of the labile molecules of ferments and toxins, bu t they are definite and even intense protoplasmic poisons. Their action is, however, not limited to the complex structures and molecules of these bodies, bu t is capable of being exerted upon much simpler and, chemically speaking, more definite substa...