The present study aims at unravelling whether an innovative, integrated method based on electron paramagnetic resonance (EPR) spectroscopy and statistical analysis could shed light the mineralogical attribution of calcium carbonate, CaCO3, microcrystalline powders found in unusual preparation layer a Renaissance painting wood. Moreover, undertaken investigation was performed to assess, if possi...

Oxalate decarboxylase (OxDC) catalyzes the Mn-dependent conversion of the oxalate monoanion into CO2 and formate. EPR-based strategies for investigating the catalytic mechanism of decarboxylation are complicated by the difficulty of assigning the signals associated with the two Mn(II) centers located in the N- and C-terminal cupin domains of the enzyme. We now report a mutational strategy that ...

The interaction of Magnesium hydrate at the phosphate oxygen atom of the pyrimidine nucleotides (CMP,UMP,dTMP) were studied at the Hartree-Fock level Theory. We used LANL2DZ basis set for Mg and 6-31g* basis set for atoms.The basis set superposition error (BSSE) begins to converge for used Method/basis set. The gauge-invariant atomic orbital (GIAO) method and the continuous-set-of-gauge-transfo...

Low temperature photolysis of nitric oxide from the nitrosyl complexes of ferric myoglobin (NO-Fe(III)Mb) and manganese(II)-porphyrin-substituted myoglobin (NO-Mn(II)Mb) was examined by electron paramagnetic resonance (EPR) spectroscopy in order to elucidate the electronic and structural natures of the photoinduced intermediates of these hemoprotein-ligand complexes trapped at low temperature. ...

Combined coordination of Zn(II) and Cu(I) or Cu(II) to the amyloid-β peptide has been investigated using XANES, EPR and NMR spectroscopies. While Zn(II) does alter Cu(II) binding to Aβ, this has no effect on (Aβ)Cu induced ROS production and associated cell toxicity.

A small soluble cytochrome c-554 purified from Methylosinus trichosporium OB3b has been purified and analyzed by amino acid sequencing, mass spectrometry, visible, CD and EPR spectroscopies. It is found to be a mono heme protein with a characteristic cytochrome c fold, thus fitting into the class of cytochrome c(2), which is the bacterial homologue of mitochondrial cytochrome c. The heme iron h...

The cover feature image illustrates two sites of a protein with different site specific reorientational dynamics (purple). Such differences can be measured by newly developed site-directed Cu(II) labeling methodology. resultant EPR lineshape at physiological temperatures is sensitive to the timescale backbone motion. Importantly, this methodology enables site-specific detection on both α-helice...

An optical Einstein-Podolsky-Rosen ~EPR! state is produced in the spontaneous parametric downconversion of an ultrashort classical pump pulse. It is shown both theoretically and experimentally that this results in the violation of a Bell-type inequality of the kind proposed by Grangier et al. The experiment is based on measuring interference between the light in the EPR state and weak light pul...