Invertase was immobilised on microporous montmorillonite K-10 via adsorption and covalent binding. The immobilised enzymes were tested for sucrose hydrolysis activity in a batch reactor. Km for immobilised systems was greater than free enzyme. The immobilised forms could be reused for 15 continuous cycles without any loss in activity. After 25 cycles, 85% initial activity was retained. A study ...

We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine d...

An enzyme immobilization technique has been developed to determine the concentration of biological compounds. This technique has been applied to penicillinase and urease, which are crosslinked as very fine films directly onto the sensitive ends of pH glass electrodes, thereby dispensing with the need of an on-line enzyme reactor. The biosensor is incorporated in an FIA system within a magnetica...

Nitrate can be removed from wastewater streams, including subsurface agricultural drainage systems, using woodchip bioreactors to promote microbial denitrification. However, the variations in water flow in these systems could make reliable performance from this microbially-mediated process a challenge. In the current work, the effects of fluctuating water levels on nitrate removal, denitrifying...

Cellulase production study was performed in shake flask and bioreactor system using Cellulomonas cellulans NRRL B 4567 for initial substrate concentration from S0 = 2 to 12 g L . The growth, substrate uptake profile and enzyme activity at different initial substrate concentrations were measured. The results inferred the presence of substrate inhibition kinetics. Various substrate inhibition mod...

The chemical system used in this communication is part of a complex molecular network described by Ashkenasy et al. [1] as an example of rational design in systems chemistry. The molecular network is engineered using nine different peptide sequences that interact via a template-directed peptide fragment condensation reaction at neutral pH. The templatedirected peptide network exhibits aspects o...