This work proposes the purification of an active and efficient enzyme, extracellular poly(3-hydroxybutyrate) (PHB)-depolymerase, suitable for industrial applications. This is achieved by the application of an easy, fast, and cheap route, skipping the chromatography step. Chromatography with one or two columns is a common step in the purification procedure, which however renders the isolation of...

Gaucher's disease is caused by a deficiency of the lysosomal enzyme glucocerebrosidase (glucosylceramidase; D-glucosyl-N-acylsphingosine glucohydrolase, EC 3.2.1.45); this disorder has been a leading candidate for enzyme replacement trials. A rapid, high-yield method for purification of glucocerebrosidase has been developed. Detergent extraction of human placenta was followed by salt fractionat...

The magnitude of the slow phase of reaction of cytochrome oxidase with cyanide has been correlated with the size of the epr signal at g' = 12. This epr signal was not found in submitochondrial particles, and significant g' = 12 epr was only observed late in the purification of solubilized enzyme. The Hartzell-Beinert procedure for the purification of cytochrome oxidase (Hartzell, C.R., and Bein...

1. The enzyme which decomposes the capsular polysaccharide of Type III Pneumococcus is associated with a protein which under optimal conditions of filtration passes through membranes with an average pore size of 10.6 mmicro but is held back by pores having a diameter of 8.2 mmicro. 2. When enzyme solutions are filtered to dryness through membranes of such porosity as to hold back the active pri...

The present study was aimed at purification and characterization of α-amylase enzyme from strain of Aspergillus niger isolated from local soil samples. Solid state fermentation (SSF) was carried out to produce α-amylase from A. niger using waste banana peel as substrate. The maximum activity of α-amylase (11926 U/gds) was recorded after 72 hours of fermentation. The extracted enzyme was subject...

In previous papers from these laboratories (1, 2) methods for the purification of the collagenase of Clostridiurm histolyticum were presented, and a calcium ion requirement for the binding of the enzyme to collagen and for the overall enzymatic action was reported. In the present, communication an additional method of purification, which makes possible the large-scale preparation of the enzyme,...

copper amine oxidases (caos) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. the enzymes are homodimers containing both topaquinone and a cu(ii) ions as cofactors at the active site of each subunit. after extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, km and vmax of th...

An enzyme which catalyzes the oxidation of L-amino acids has been isolated from silkworm eggs (Bombyx mori L.), and purified. The activity of the enzyme preparation is about 1584 times more than that of the crude preparation. Methods of purification of the enzyme are described in detail and certain physicochemical properties of the enzyme are also described.

The harvest media from five strains of Pseudomonas aeruginosa collected from severe clinical infections did not contain a collagenase. Instead, an enzyme was isolated that could degrade the ground substance of the cornea. Partial purification of this enzyme indicated that it was the only enzyme in the harvest media that cotdd destroy the rabbit cornea after intralamellar injection. Na2EDTA comp...

Purification of cyclic 3’,5’-nucleotide phosphodiesterase of bovine brain cerebrum resulted in partial loss of activity, due to dissociation of an activator (or cofactor) from the enzyme. A systematic study showed that as purification proceeded, the activator was removed from phosphodiesterase in a stepwise fashion. Fractions representing phosphodiesterase at different stages of purification co...